https://eln.excellgen.com/index.php?action=history&feed=atom&title=1602_pL 1602 pL - Revision history 2026-10-18T16:43:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1602_pL&diff=8210&oldid=prev Paul.xu at 18:37, 13 February 2013 2013-02-13T18:37:45Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of ‎1602 pL&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1602 pL <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-2-7--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1602&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> === PCR ===<br /> <br /> gene synthesis=&amp;gt;150bp&lt;br&gt; <br /> <br /> [[Image:1602PCR 2 7 2013.JPG]]&lt;br&gt; <br /> <br /> Cut out the band, digest PCR products and&amp;nbsp;1279 pT7A3with BglII + Xba1&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 1602.JPG]]&lt;br&gt; <br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width: 281px; height: 66px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 3&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 11279&lt;br&gt; <br /> | PCR&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp; BglII + Xba1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013- <br /> <br /> Make miniprep and digest with BglII + Xba1 <br /> <br /> [[Image:1602DIGEST.JPG‎]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;lt;br&amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu