https://eln.excellgen.com/index.php?action=history&feed=atom&title=1608-pT7A3-K8XHis-MBP-%283C%29 1608-pT7A3-K8XHis-MBP-(3C) - Revision history 2026-10-18T17:28:53Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1608-pT7A3-K8XHis-MBP-(3C)&diff=7870&oldid=prev Paul.xu at 18:41, 16 January 2013 2013-01-16T18:41:58Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1608-pT7A3-K8XHis-MBP-(3C)&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1608-pT7A3-K8XHis-MBP-(3C) <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-10---2013-1-16<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1608&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1608+1610+1611+1612+1613 pcr 1-10-2013.JPG]]&lt;br&gt; <br /> <br /> [[Image:1608+1610+1611 clone 1-14-2013.JPG]][[Image:1608 clone 1-14-2013.JPG]]&lt;br&gt; <br /> <br /> lane 1.Digest PCR product with BspH1 + Nde1, &lt;br&gt; <br /> <br /> lane 2.clone into1279pT7A3 vector&#039;s Nco1 + Nde1 sites.&lt;br&gt;&lt;br&gt; <br /> <br /> <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-1-16 <br /> <br /> Make miniprep and digest with Xba1 + Nde1<br /> <br /> [[Image:1608+1610_digest_detect_1-16-2013.JPG]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;lt;br&amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;amp;lt;br&amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;amp;lt;br&amp;amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu