https://eln.excellgen.com/index.php?action=history&feed=atom&title=1611-pCold-MBP 2026-10-18T06:18:41Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1611-pCold-MBP&diff=8272&oldid=prev 2013-02-18T18:32:54Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1611-pCold-MBP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1611-pCold-MBP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-10---2013-1-16 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1611&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1608+1610+1611+1612+1613 pcr 1-10-2013.JPG]]&lt;br&gt; <br /> <br /> [[Image:1611 PCR 1-11-2013.JPG]]&lt;br&gt; <br /> <br /> [[Image:1608+1610+1611 clone 1-14-2013.JPG]]&lt;br&gt; <br /> <br /> lane 5. digest PCR product with BglII + Nde1 <br /> <br /> lane 6. Digest vector with BglII + Nde1.&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-1-16 <br /> <br /> Make miniprep and digest with BglII + Nde1.&lt;br&gt; <br /> <br /> [[Image:1611 digest detect 1-16-2013.JPG]] <br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1611-pCold-MBP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1611-pCold-MBP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-25--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1611&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> 2012/1/25 <br /> <br /> pcr1 <br /> <br /> gene synthesis&lt;br&gt; <br /> <br /> [[Image:1611pcr1+1427+1429pcr1 1-25-2013.jpg]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> pcr2 <br /> <br /> [[Image:1608+1610+1611+1612+1613 pcr 1-10-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> .pcr1+pcr2 <br /> <br /> [[Image:1611+1614+1615+1629 pcr 1-28-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1611+1614+1615+1629 clone 1-29-2013.JPG]]&lt;br&gt; <br /> <br /> digest PCR product with&amp;nbsp; BamH1 + Nde1 <br /> <br /> 5. Digest vector with BglII + Nde1&lt;br&gt; <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013- <br /> <br /> Make miniprep and digest with &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Cloning of 1611-pCold-MBP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1611-pCold-MBP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-2-14--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1611&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> 2012/1/25 <br /> <br /> pcr1 <br /> <br /> Method 1: PCR promoter from v19 pET28-cold===&lt;br&gt;Cold-BamH1-F &lt;br&gt;Cold-8 &lt;br&gt; <br /> <br /> gene synthesis&lt;br&gt; <br /> <br /> [[Image:1611+1614 pcr1 2-14-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> pcr2 <br /> <br /> [[Image:1608+1610+1611+1612+1613 pcr 1-10-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> .pcr1+pcr2 <br /> <br /> [[Image:1611+1614pcr2 2-15-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> digest PCR product with&amp;nbsp; BamH1 + Nde1 <br /> <br /> . Digest vector with BglII + Nde1&lt;br&gt; <br /> <br /> [[Image:1611 clone 2-15-2013.JPG]] <br /> <br /> <br /> <br /> === Colony Screening ===<br /> <br /> 2013/2/18<br /> <br /> Make miniprep and digest with BglII + Nde1<br /> <br /> [[Image:1674_1611_2013_2_18_2.JPG]][[Image:1674_1611_2013_2_18_1.JPG]]<br /> <br /> clone 1, 3, 4, 5 may be OK. Send #1 to sequencing<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> <br /> <br /> [[Category:Cloning]]</div>

Paul.xu