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1615-pLVX-EF1-G93A-SOD1-puro - Revision history
2026-10-18T05:04:09Z
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Paul.xu at 17:54, 31 January 2013
2013-01-31T17:54:41Z
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<p><b>New page</b></p><div>== '''Cloning of 1615-pLVX-EF1-G93A-SOD1-puro''' ==<br />
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'''Purpose:''' Cloning of 1615-pLVX-EF1-G93A-SOD1-puro <br />
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'''Date: '''2013-1-28---2013-1-31<br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1615''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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=== [[Image:1614+1615 pcr1-2 1-28-2013.JPG]] ===<br />
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pcr1+pcr2<br> <br />
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[[Image:1611+1614+1615+1629 pcr 1-28-2013.JPG]] <br />
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[[Image:1611+1614+1615+1629 clone 1-29-2013.JPG]]<br> <br />
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Digest pcr product with EcoR1 + Xho1. <br />
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Clone into EcoR1 + Xho1 digested 42 pLVX-EF1-MCS-Puro<br> <br />
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=== Colony Screening ===<br />
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Date: 2013-1-31<br />
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Make miniprep and digest with EcoR1 + Xho1<br> <br />
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[[Image:1615_digest_detect_1-31-2013.JPG]]<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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*&amp;amp;amp;lt;br&amp;amp;amp;gt;<br />
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== '''Conclusion''' ==<br />
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&amp;amp;amp;lt;br&amp;amp;amp;gt; <br />
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[[Category:Cloning]]</div>
Paul.xu