https://eln.excellgen.com/index.php?action=history&feed=atom&title=1621-pAAV-PR8-HAin 1621-pAAV-PR8-HAin - Revision history 2026-10-18T17:25:06Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1621-pAAV-PR8-HAin&diff=7964&oldid=prev Paul.xu at 20:56, 24 January 2013 2013-01-24T20:56:24Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1621-pAAV-PR8-HAin&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1621-pAAV-PR8-HAin <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-1-22---2013-1-24<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1621&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1620+1621 clone 1-22-2013.JPG]] <br /> <br /> digest 1463 pAD5-PR8-HAin using Sal1 + BamH1&lt;br&gt;digest vector 254 pAAV.CMV-MCS, pAAV-MCS with Xho1 + BamH1&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-1- <br /> <br /> Make miniprep and digest with EcoR1&lt;br&gt;<br /> <br /> [[Image:1620+1621_digest_detect_1-24-2013.JPG]]<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu