https://eln.excellgen.com/index.php?action=history&feed=atom&title=1631_pT7A3-ID3 1631 pT7A3-ID3 - Revision history 2026-10-18T15:05:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1631_pT7A3-ID3&diff=10931&oldid=prev Paul.xu: Created page with "== '''Cloning of 1631 pT7A3-ID3''' == '''Purpose:''' Cloning of1631 pT7A3-ID3 '''Date: '''2014-1---- '''Notebook:0''' '''Page:''' '''Clone ID:1631''' '''Experimenters..." 2014-01-13T18:16:21Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1631 pT7A3-ID3&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1631 pT7A3-ID3 &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1631&#039;&#039;&#039; &#039;&#039;&#039;Experimenters...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1631 pT7A3-ID3&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of1631 pT7A3-ID3 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-1---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1631&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR from 1260 pLenti6.2-ID3&lt;br&gt;ID3-Nde1-Nhe1-F&lt;br&gt;cgatcgCATATG GCTAGC AAGGCGCTGAGCCCGGTG&lt;br&gt;ID3-HindIII-R&lt;br&gt;tgctagAAGCTTCtAGTGGCAAAAGCTCCTTTTGTC&lt;br&gt;<br /> <br /> [[Image:1561_1631_1693PCR.JPG]]&lt;br&gt;<br /> <br /> digest with Nde1 + HindIII, ligate to Nde1 + HindIII digested 1279 pT7A3&lt;br&gt;<br /> <br /> [[Image:Digest_pcrfor_1561_1631_1693_1942_1995.JPG]]&lt;br&gt;<br /> <br /> [[Image:2digest_vectors_for_1561_1631_1693_1942_1995.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> Make miniprep, digest with NdeI+HindIII&lt;br&gt; <br /> <br /> [[Image:1561_1631_1693_1942_1995digest.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu