https://eln.excellgen.com/index.php?action=history&feed=atom&title=1636_1545_1544_1543_1992_1680_1694_2092 1636 1545 1544 1543 1992 1680 1694 2092 - Revision history 2026-10-18T11:42:05Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1636_1545_1544_1543_1992_1680_1694_2092&diff=13466&oldid=prev Excellgen at 13:10, 23 October 2014 2014-10-23T13:10:26Z <p></p> <p><b>New page</b></p><div>== Protein Expression ==<br /> <br /> 2014/6/4&lt;br&gt; SHANYI use 20ul magnetic beads <br /> <br /> [[Image:1636 1545 1544 1543 1992 1680 1694 2092.jpg]]&lt;br&gt; <br /> <br /> {| width=&quot;832&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | SAMPLE&lt;br&gt; <br /> | pI/Mw&lt;br&gt; <br /> | express&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pT7A3-VVCap-S-GGGS(with 3X GGGS linker)&lt;br&gt; <br /> | rowspan=&quot;1&quot; | VVCap-S-GGGS&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> 1636&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | <br /> 15 ml, <br /> <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.3mM IPTG RT O/N&lt;br&gt; <br /> | s&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> 8.67/38&lt;br&gt; <br /> <br /> | NO, cell died&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pT7A3-VVCap&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1545&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.3mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 8.85/72&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; 1545&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.3mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | p&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pT7A3-VVCap-L(large subunit)&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1544&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.3mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 8.74/36.9&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pT7A3-VVCap-S(small subunit )&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1543&lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.3mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 8.82/33.4&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pL42-SM2&lt;br&gt; <br /> | rowspan=&quot;1&quot; | Monomer Benzonase H184R&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> &lt;br&gt; 1992&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.3mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 6.54/27.9&lt;br&gt; <br /> | no &lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | <br /> pageRuler Plus Prestained protein ladder&lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pCold-GST-wtSM2&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; 1680&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.3mM IPTG RT O/N&lt;br&gt; <br /> | s&lt;br&gt; <br /> | <br /> 6.18/60.3&lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pT7A3-wtSM2&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1694&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> &amp;nbsp;15 ml, <br /> <br /> Nico21(DE3)plysN &lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.3mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | &lt;br&gt; &lt;br&gt; <br /> | &lt;br&gt; <br /> | 1694&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.3mM IPTG RT O/N&lt;br&gt; <br /> | p&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | pT7A3-MBP-i3C-6H&lt;br&gt; <br /> | &lt;br&gt; <br /> | 2092&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | 0.3mM IPTG RT O/N&lt;br&gt; <br /> | S&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 12&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | 2092&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | p&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> need to redo <br /> <br /> pL42 vector: induce at 42 oC for 2~3 hours, grow at 30 oc&lt;br&gt; <br /> <br /> 1680, 1694 with C-CBD, NO his tag&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Excellgen