https://eln.excellgen.com/index.php?action=history&feed=atom&title=1657-pCold-6His-GST-%28E%29-%283C%29-sfGFP 1657-pCold-6His-GST-(E)-(3C)-sfGFP - Revision history 2026-10-18T12:06:09Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1657-pCold-6His-GST-(E)-(3C)-sfGFP&diff=8565&oldid=prev Paul.xu at 16:55, 14 March 2013 2013-03-14T16:55:43Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1657-pCold-6His-GST-(E)-(3C)-sfGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1519-pT7A3-T3-DNA-Ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-2-14---2013-2-21 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1657&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR sfGFP from 1005 pBad24-Tat-sfGFP using &lt;br&gt;sfGFP-Kpn1-Nhe1-Sal1-F&lt;br&gt;sfGFP-HindIII-Spe1-Not1-Xho1-R&lt;br&gt; <br /> <br /> [[Image:1584+1657 pcr 2-14-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1657 clone 2-19-2013.JPG]] <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-2-21 <br /> <br /> Make miniprep and digest with Nhe1+Xho1&lt;br&gt; <br /> <br /> [[Image:1657 digest detect 2-21-2013.JPG]] <br /> <br /> &lt;br&gt;<br /> <br /> &#039;&#039;&#039;NO sfGFP ,parent vector, 1624 pCold-6His-GST-(E)-(3C)&#039;&#039;&#039; has two Nhe1 sites&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu