https://eln.excellgen.com/index.php?action=history&feed=atom&title=1661_1674_1683 1661 1674 1683 - Revision history 2026-10-18T03:12:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1661_1674_1683&diff=9277&oldid=prev Paul.xu: Created page with "== 1661 1674 1683<br> == == Protein Expression == <br> 2013/3/5 Image:1661 1674 1683 1676 3 6 2013.jpg {| cellspacing="1" cellpadding="1" border="1" width="684" |- ..." 2013-05-29T19:15:08Z <p>Created page with &quot;== 1661 1674 1683&lt;br&gt; == == Protein Expression == &lt;br&gt; 2013/3/5 <a href="/index.php?title=File:1661_1674_1683_1676_3_6_2013.jpg" title="File:1661 1674 1683 1676 3 6 2013.jpg">Image:1661 1674 1683 1676 3 6 2013.jpg</a> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;684&quot; |- ...&quot;</p> <p><b>New page</b></p><div>== 1661 1674 1683&lt;br&gt; ==<br /> <br /> == Protein Expression ==<br /> <br /> &lt;br&gt; <br /> <br /> 2013/3/5 <br /> <br /> [[Image:1661 1674 1683 1676 3 6 2013.jpg]] <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;684&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | Purification&lt;br&gt;<br /> |-<br /> | M&lt;br&gt; <br /> | Fermentas PageRuler Plus&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | pT7A3-MBP-(3c)-NRF2 &lt;br&gt; <br /> | <br /> 1661 <br /> <br /> clone 1&lt;br&gt; <br /> <br /> | <br /> 4 ml,&lt;br&gt; <br /> <br /> BL21(DE3)Star, pRare2-pLysS <br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | pT7A3-MBP-(3c)-KEAP1&lt;br&gt; <br /> | <br /> 1661 <br /> <br /> Clone 2&lt;br&gt; <br /> <br /> | <br /> 4 ml, <br /> <br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | pCold-GST-(3c)-NRF2&lt;br&gt; <br /> | 1674&lt;br&gt; <br /> | <br /> 4 ml, <br /> <br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads (wrong, should use Glutathione agarose beads, no his tag)&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | pCold-GST-(3c)-NRF2&lt;br&gt; <br /> | 1674&lt;br&gt; <br /> | <br /> 4 ml, <br /> <br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads (wrong, should use Glutathione agarose beads, no his tag)&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | pCold-MBP-(3C)-NRF2&lt;br&gt; <br /> | 1683&lt;br&gt; <br /> | <br /> 4 ml, <br /> <br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | pCold-MBP-(3C)-NRF2&lt;br&gt; <br /> | 1683&lt;br&gt; <br /> | 4 ml,BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | pT7A3-MBP-(3c)-KEAP1&lt;br&gt; <br /> | 1625&lt;br&gt; <br /> | 4 ml,BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | pCold-MBP-(3C)-KEAP1&lt;br&gt; <br /> | 1676&lt;br&gt; <br /> | 4 ml,BL21(DE3)Star, pRare2-pLysS &lt;br&gt; <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt; <br /> | Ni beads&lt;br&gt;<br /> |-<br /> | 9 <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 10 <br /> | Purified MBP <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu