https://eln.excellgen.com/index.php?action=history&feed=atom&title=1661_1682_1683_1719_1748_1752_1771_1779 1661 1682 1683 1719 1748 1752 1771 1779 - Revision history 2026-10-18T16:42:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1661_1682_1683_1719_1748_1752_1771_1779&diff=9633&oldid=prev Paul.xu at 14:49, 8 July 2013 2013-07-08T14:49:45Z <p></p> <p><b>New page</b></p><div>Date:2013-7-5&lt;br&gt; <br /> <br /> [[Image:1661+1682+1683+1719+1748+1752+1771+1779protein_screening_7-5-2013.JPG]]&lt;br&gt;<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;200&quot;<br /> |-<br /> | Lane&lt;br&gt;<br /> | Name&lt;br&gt;<br /> | Host&lt;br&gt;<br /> | Induction&lt;br&gt;<br /> | Purification&lt;br&gt;<br /> | MW&lt;br&gt;<br /> | Conclusion&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt;<br /> | 1661,pT7A3-MBP-(3c)-NRF2&lt;br&gt;<br /> | 4 ml,<br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt;<br /> | Ni beads&lt;br&gt;<br /> | 112KD&lt;br&gt;<br /> | yes&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt;<br /> | 1682, pCold-6His-GST-(E)-(3C)-NRF2&lt;br&gt;<br /> | 4 ml,<br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt;<br /> | Ni beads&lt;br&gt;<br /> | 97KD&lt;br&gt;<br /> | yes&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt;<br /> | 1683,pCold-MBP-(3C)-NRF2&lt;br&gt;<br /> | 4 ml,<br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt;<br /> | Ni beads&lt;br&gt;<br /> | 113KD&lt;br&gt;<br /> | yes&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt;<br /> | 1719,T7A3-MBP-(E3C)-NRF2&lt;br&gt;<br /> | 4 ml,<br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt;<br /> | Ni beads&lt;br&gt;<br /> | 112KD&lt;br&gt;<br /> | no&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt;<br /> | 1748,pCold-8His-MBP-NRF2-6His&lt;br&gt;<br /> | 4 ml,<br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt;<br /> | Ni beads&lt;br&gt;<br /> | 120KD&lt;br&gt;<br /> | no&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt;<br /> | 1752,pCold-TF-(E3C)-NRF2&lt;br&gt;<br /> | 4 ml,<br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt;<br /> | Ni beads&lt;br&gt;<br /> | 120KD&lt;br&gt;<br /> | yes&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt;<br /> | 1771,pT7A3-MBP-(3C)-PCK1&lt;br&gt;<br /> | 4 ml,<br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt;<br /> | Ni beads&lt;br&gt;<br /> | 110KD&lt;br&gt;<br /> | yes&lt;br&gt;<br /> |-<br /> | 8&lt;br&gt;<br /> | 1779, pTCold-MBP-(3C-TEV)-NRF2&lt;br&gt;<br /> | 4 ml,<br /> NiCO21(DE3), pRare2-pLysS &lt;br&gt;<br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt;<br /> | Ni beads&lt;br&gt;<br /> | 113KD&lt;br&gt;<br /> | yes&lt;br&gt;<br /> |-<br /> | 9&lt;br&gt;<br /> | 1779, pTCold-MBP-(3C-TEV)-NRF2&lt;br&gt;<br /> | 4 ml,<br /> BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> <br /> | 0.2 mM IPTG 25 oC o/n&lt;br&gt;<br /> | Ni beads&lt;br&gt;<br /> | 113KD&lt;br&gt;<br /> | yes&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu