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1662-pHot - Revision history
2026-10-18T11:18:43Z
Revision history for this page on the wiki
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Paul.xu at 16:27, 17 July 2013
2013-07-17T16:27:27Z
<p></p>
<p><b>New page</b></p><div>== '''Cloning of 1662-pHot''' ==<br />
<br />
'''Purpose:''' Cloning of 1662-pHot <br />
<br />
'''Date: '''2013-3-5---2013-3-7 <br />
<br />
'''Notebook:0''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:1662''' <br />
<br />
'''Experimenters:''' yingying <br />
<br />
== '''Results''' ==<br />
<br />
=== cloning ===<br />
<br />
[[Image:1542+1662+1673 clone 3-5-2013.JPG]] <br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2013-3-7 <br />
<br />
Make miniprep and digest with Nde1 + Xho1<br> <br />
<br />
[[Image:1662 digest detect 3-7-2013.JPG]]<br> <br />
<br />
Comment by Paul: This clone is Junk! <br />
<br />
Can not be sequened, and the gel picture shows this is a junk clone <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
<br> <br />
<br />
== '''Conclusion''' ==<br />
<br />
== '''Cloning of 1662-pHot''' ==<br />
<br />
'''Purpose:''' Cloning of 1662-pHot <br />
<br />
'''Date: '''2013-7 <br />
<br />
'''Notebook:0''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:1662''' <br />
<br />
'''Experimenters:''' shanyi <br />
<br />
== '''Results''' ==<br />
<br />
=== cloning ===<br />
<br />
digest vector1658 pCP40 with EcoR1 + Xba1,Ligate to annealed oligos <br />
<br />
EcoR1-MCS-Xba1-T + EcoR1-MCS-Xba1-B<br> <br />
<br />
<br> <br />
<br />
new MCS Nco1 Nhe1 BamH1 EcoR1 HindIII Not1 Xho1 Xba1<br>AATTTAAGAAGGAGATATACC ATG GGC GCT AGC GGA TCC GAA TTC AAGCTT GCGGCCGCACTCGAG T<br> aTTCTTCCTCTATATGG TAC CCG CGA TCG CCT AGG CTT AAG TTCGAA CGCCGGCGTGAGCTC AGATC <br />
<br />
[[Image:Digest for 1773 1775 1662.JPG]] <br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2013-3-7 <br />
<br />
Make miniprep and digest with Nde1 + Xho1<br> <br />
<br />
[[Image:1658DIGEST WITH NdeI+XhoI.JPG]][[Image:1662digest.JPG]]<br> <br />
<br />
sent 1662-1 to sequence,no priming<br> <br />
<br />
== '''Redo Cloning of 1662-pHot''' ==<br />
<br />
'''Purpose:''' Cloning of 1662-pHot <br />
<br />
'''Date: '''2013-7 <br />
<br />
'''Notebook:0''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:1662''' <br />
<br />
'''Experimenters:''' shanyi <br />
<br />
== '''Results''' ==<br />
<br />
=== cloning ===<br />
<br />
digest vector1658 pCP40 with EcoR1 + Xba1,Ligate to annealed oligos <br />
<br />
EcoR1-MCS-Xba1-T + EcoR1-MCS-Xba1-B<br> <br />
<br />
<br> <br />
<br />
new MCS Nco1 Nhe1 BamH1 EcoR1 HindIII Not1 Xho1 Xba1<br>AATTTAAGAAGGAGATATACC ATG GGC GCT AGC GGA TCC GAA TTC AAGCTT GCGGCCGCACTCGAG T<br> aTTCTTCCTCTATATGG TAC CCG CGA TCG CCT AGG CTT AAG TTCGAA CGCCGGCGTGAGCTC AGATC <br />
<br />
[[Image:Digest for 1773 1775 1662.JPG]] <br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2013-7-17 <br />
<br />
Make miniprep and digest with Nde1 + Xho1<br> <br />
<br />
[[Image:1662digest2.JPG ]]<br> <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
<br> <br />
<br />
== '''Conclusion''' ==<br />
<br />
[[Category:Cloning]]</div>
Paul.xu