https://eln.excellgen.com/index.php?action=history&feed=atom&title=1666-pT7A3-GST-%28E3C%29 1666-pT7A3-GST-(E3C) - Revision history 2026-10-18T17:27:47Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1666-pT7A3-GST-(E3C)&diff=9385&oldid=prev Paul.xu at 15:39, 7 June 2013 2013-06-07T15:39:49Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1666-pT7A3-GST-(E3C)&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1666-pT7A3-GST-(E3C) <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-2-22----2013-2-25 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1666&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1664+1666+1667 clone 2-22-2013.JPG]] <br /> <br /> digest vector 1665 pT7A3-GST-(TEV) with Nde1 + BamH1,&lt;br&gt;ligate to annealled oligos Nde1-3C-BamH1-T + Nde1-3C-BamH1-B&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-2-25 <br /> <br /> Make miniprep and digest with:Xba1 + Pst1 <br /> <br /> [[Image:1666+1678 digest detect 2-25-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> <br /> [[Category:Cloning]]</div> Paul.xu