https://eln.excellgen.com/index.php?action=history&feed=atom&title=1673_1710_2267_2324_2332_2343_2345_637 1673 1710 2267 2324 2332 2343 2345 637 - Revision history 2026-10-18T03:14:18Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1673_1710_2267_2324_2332_2343_2345_637&diff=13317&oldid=prev Excellgen: Created page with "== Protein Expression == 2014/9/<br> SHANYI Image:1673_1710_2267_2324_2332_2343_2345_637.jpg<br> <br> {| width="832" cellspacing="1" cellpadding="1" border="1" |- | Lan..." 2014-09-30T13:14:43Z <p>Created page with &quot;== Protein Expression == 2014/9/&lt;br&gt; SHANYI <a href="/index.php?title=File:1673_1710_2267_2324_2332_2343_2345_637.jpg" title="File:1673 1710 2267 2324 2332 2343 2345 637.jpg">Image:1673_1710_2267_2324_2332_2343_2345_637.jpg</a>&lt;br&gt; &lt;br&gt; {| width=&quot;832&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; |- | Lan...&quot;</p> <p><b>New page</b></p><div>== Protein Expression ==<br /> <br /> 2014/9/&lt;br&gt; SHANYI <br /> <br /> [[Image:1673_1710_2267_2324_2332_2343_2345_637.jpg]]&lt;br&gt; &lt;br&gt; <br /> <br /> {| width=&quot;832&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | SAMPLE&lt;br&gt; <br /> | pI/Mw&lt;br&gt; <br /> | express&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pET28b-NdeI&lt;br&gt; <br /> | rowspan=&quot;1&quot; | NdeI&lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1673&lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> 15 ml, <br /> <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.4mM IPTG RT O/N&lt;br&gt; <br /> | s&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 8.6/46.4&lt;br&gt; <br /> | &amp;nbsp; yes&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pET28b-NdeI&lt;br&gt; <br /> | rowspan=&quot;1&quot; | NdeI&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1673&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Tuner(DE3)plysN-ha-plac-hia5&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.4mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | yes&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pT7A3-NdeI&lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; NdeI&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1710&lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.2mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | S&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | yes&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pT7A3-NdeI&lt;br&gt; <br /> | rowspan=&quot;1&quot; | NdeI&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 1710&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Tuner(DE3)plysN-ha-plac-hia5&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.2mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | YES&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt;<br /> | rowspan=&quot;1&quot; | &lt;br&gt;&lt;br&gt; <br /> | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pT7A3-Tth-PPase &lt;br&gt;&lt;br&gt; <br /> | rowspan=&quot;1&quot; | Tth-PPase &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 2267&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.2mM IPTG RT O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 5.6/20.9&lt;br&gt; <br /> | yes&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | <br /> pL6H-sso7d-wt-Taq&lt;br&gt; <br /> <br /> | sso7d-wt-Taq&lt;br&gt; <br /> | 2324&lt;br&gt; <br /> | 15 ml, <br /> Nico21(DE3)plysN &amp;nbsp; &lt;br&gt; <br /> <br /> | 42oC O/N&lt;br&gt;&lt;br&gt; <br /> | s&lt;br&gt; <br /> | <br /> 6.26/104.7&lt;br&gt; <br /> <br /> | YES, 42oC O/N inducing is too long, cell lysed&lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pT7A30-Cas9&lt;br&gt; <br /> | rowspan=&quot;1&quot; | &lt;br&gt; Cas9&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 2332&lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml,<br /> Nico21(DE3)plysN &lt;br&gt;<br /> <br /> | rowspan=&quot;1&quot; | 0.2mM IPTG RT O/N&lt;br&gt; <br /> | s&lt;br&gt; <br /> | <br /> 9.0/164&lt;br&gt; <br /> <br /> | yes&lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | rowspan=&quot;1&quot; | pET28b-T4-endo-VII&lt;br&gt; <br /> | rowspan=&quot;1&quot; | T4-endo-VII&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 2343&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Nico21(DE3)plysN &lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.2mM IPTG RT O/N&lt;br&gt; <br /> | S&lt;br&gt; <br /> | <br /> 8.9/20.3&lt;br&gt; <br /> <br /> | yes&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | &lt;br&gt; pCold-HCMVUL98&lt;br&gt; <br /> | HCMVUL98&lt;br&gt; &lt;br&gt; <br /> | 2345&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> 15 ml,<br /> <br /> Nico21(DE3)plysN&lt;br&gt; <br /> <br /> | rowspan=&quot;1&quot; | 0.2mM IPTG RT O/N&lt;br&gt; <br /> | S&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 6..03/67.1&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | pT7A3-NcoI&lt;br&gt;<br /> | &lt;br&gt; <br /> | 637&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Tuner(DE3)plysN-ha-plac-hia5&lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | 0.2mM IPTG RT O/N&lt;br&gt; <br /> | S&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 5.6/31.5&lt;br&gt; <br /> | no&lt;br&gt;<br /> |-<br /> | 12&lt;br&gt; <br /> | pT7A3-NcoI&lt;br&gt; <br /> | &lt;br&gt; <br /> | 637&lt;br&gt; <br /> | rowspan=&quot;1&quot; | 15 ml, <br /> Top10plysN-ha-plac-hia5&lt;br&gt; <br /> <br /> | S&lt;br&gt; <br /> | 5.6/31.5&lt;br&gt; <br /> | no&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Excellgen