https://eln.excellgen.com/index.php?action=history&feed=atom&title=167_pT7A3-MBP-TRX-6H 2026-10-18T20:11:56Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=167_pT7A3-MBP-TRX-6H&diff=19761&oldid=prev 2019-01-01T18:47:36Z

<p></p> <p><b>New page</b></p><div>to remove NcoI site, <br /> <br /> 167 two BsaI sites.&lt;br/&gt;Clone annealled oligos into Xba1-Nco1 digested parental vector 2375&lt;br/&gt;2094-T&lt;br/&gt;CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAT&lt;br/&gt;&amp;nbsp; &amp;nbsp; TTTATTAAAACAAATTGAAATTCTTCCTCTATATAGTAC&lt;br/&gt;2094-B&lt;br/&gt;CATGATATATCTCCTTCTTAAAGTTAAACAAAATTATTT<br /> <br /> [[File:167 digestion.JPG|RTENOTITLE]]<br /> <br /> [[File:167 161 316 160 digestion.JPG|RTENOTITLE]]<br /> <br /> [[File:316 167 digestion.JPG|RTENOTITLE]]<br /> <br /> 167 #2 is good, sequenced<br /> [[Category:Cloning]]</div>

Paul.xu