https://eln.excellgen.com/index.php?action=history&feed=atom&title=169-pET28b-ssfGFP 2026-10-18T11:51:53Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=169-pET28b-ssfGFP&diff=2923&oldid=prev 2012-01-03T20:31:32Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 169-pET28b-ssfGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 169-pET28b-ssfGFP by gene synthesis&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-12-28 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:169&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;[[Image:169 PCR 12-29-2011.JPG]]&#039;&#039;&#039;&lt;br&gt; <br /> <br /> &#039;&#039;&#039;gene synthesis, mkate1 + ssfGFP19, using these mixture: ssfGFP1&lt;br&gt;ssfGFP2&lt;br&gt;ssfGFP3&lt;br&gt;ssfGFP4&lt;br&gt;ssfGFP5&lt;br&gt;ssfGFP6&lt;br&gt;ssfGFP7&lt;br&gt;ssfGFP8&lt;br&gt;ssfGFP9&lt;br&gt;ssfGFP10&lt;br&gt;ssfGFP11&lt;br&gt;ssfGFP12&lt;br&gt;ssfGFP13&lt;br&gt;ssfGFP14&lt;br&gt;ssfGFP15&lt;br&gt;ssfGFP16&lt;br&gt;ssfGFP17&lt;br&gt;ssfGFP18&lt;br&gt;ssfGFP19&#039;&#039;&#039; <br /> <br /> &lt;span style=&quot;font-weight: bold;&quot;&gt;Primer-F mkate1, 2010_07_07_Paul Xu_5909785_3602904.XLS<br /> Primer-R ssfGFP19&lt;/span&gt;&lt;br&gt; <br /> <br /> [[Image:169+323+459 clone 12-29-2011.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 367px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | pET28b&lt;br&gt;<br /> |-<br /> | digest enzyme&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; BamH1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === subcloning ===<br /> <br /> Date:&amp;nbsp; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-1-3&lt;br&gt; <br /> <br /> Make miniprep and digest with BamH1 + Xho1&lt;br&gt; <br /> <br /> [[Image:169+323_digest_detect_1-3-2012.JPG]]<br /> <br /> clone 1,3,4,5,6 may be right, need to sequence <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;nbsp;&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *Sequencing Result:&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> &lt;br&gt; --[[User:Yingying.lu|Yingying.lu]] 16:03, 29 December 2011 (EST) <br /> <br /> [[Category:Cloning]]</div>

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