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1696 1707 1567 1673 1697 - Revision history
2026-10-18T02:19:55Z
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Paul.xu at 14:31, 8 October 2018
2018-10-08T14:31:07Z
<p></p>
<p><b>New page</b></p><div><br/>2013/4/5<br />
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[[File:1696 1707 1567 1673 1697 protein 4-5-2013.JPG|RTENOTITLE]]<br />
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{| border="1" width="200" cellspacing="1" cellpadding="1"<br />
|-<br />
| lane<br/><br />
| 1<br />
| 2<br />
| 3<br />
| 4<br />
| 5<br />
| 6<br />
| 7<br />
| 8<br/><br />
| 9<br/><br />
| 10<br/><br />
|-<br />
| clone ID<br />
| colspan="5" | <br/>1696-pCold-MBP-(3C)-TEV<br />
| colspan="2" | <br/>1707-pCold-MBP-(3C)-TEV<br />
| 1567pT7A3-T5-Exo<br/><br />
| 1673-pET28b-Nde1<br/><br />
| 1697-pCold-GST-(3c)-wt3C<br/><br />
|-<br />
| host<br/><br />
| colspan="5" | <br/>Nico21DE3plysN<br/><br />
| colspan="2" | <br/>BL21DE3pRare2pLysS<br/><br />
| Nico21DE3plysN<br/><br />
| Nico21DE3plysN<br/><br />
| Nico21DE3plysN<br/><br />
|-<br />
| induction<br/><br />
| colspan="5" | 0.2 mM IPTG 25 oC o/n<br/><br />
| colspan="2" | 0.2 mM IPTG 25 oC o/n<br/><br />
| 0.2 mM IPTG 25 oC o/n<br/><br />
| 0.2 mM IPTG 25 oC o/n<br/><br />
| 0.2 mM IPTG 25 oC o/n<br/><br />
|-<br />
| purification<br/><br />
| colspan="5" | 5ml HisTrap HP<br/><br />
| colspan="2" | 5ml HisTrap HP<br/><br />
| Ni beads<br/><br />
| Ni beads<br/><br />
| Ni beads<br/><br />
|-<br />
| MW<br/><br />
| colspan="5" | 72KD/6.04<br/><br />
| colspan="2" | 88KD/5.76<br/><br />
| 33KD/5.22<br/><br />
| 44KD/8.41<br/><br />
| 21KD/8.47<br/><br />
|}<br />
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[[Category:Protein Purification]]<br />
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PageRuler Plus 250, 130, 100, 70(red) 55, 35, 25 (red), 15, 10</div>
Paul.xu