https://eln.excellgen.com/index.php?action=history&feed=atom&title=1701-pMAL-c5E-KEAP1 2026-10-18T07:29:36Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1701-pMAL-c5E-KEAP1&diff=9302&oldid=prev 2013-05-29T19:50:11Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1701-pMAL-c5E-KEAP1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1701-pMAL-c5E-KEAP1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-3-8---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1701&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> [[Image:1701 clone 3-8-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013/3/9 <br /> <br /> Make miniprep and digest with Nde1 + HindIII&lt;br&gt; <br /> <br /> [[Image:1701 Nde1 HindIII 2013 3 9.jpg]]&lt;br&gt; <br /> <br /> digest clone #4 with EcoR1<br /> <br /> [[Image:1701_EcoR1_2013_3_9.JPG]]<br /> <br /> Transform clone Bl21 Star (DE3)/pRare2pLysS with #4, grow on LB/amp/chl plate<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> == Protein expression of KEAP1 clones&lt;br&gt; ==<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;415&quot;<br /> |-<br /> | clone ID&lt;br&gt; <br /> | expression&lt;br&gt; <br /> | ge image&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1625,pT7A3-MBP-(3c)-KEAP1&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | [[1625 1626 1656]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1656,pCold-GST-(3c)-KEAP1&lt;br&gt; <br /> | need to use GST beads&lt;br&gt; <br /> | [[1625 1626 1656]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1676,pCold-MBP-(3C)-KEAP1&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | [[1661 1674 1683]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1701,pMAL-c5E-KEAP1&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1720,pT7A3-MBP-(E3C)-Keap1&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[626 1717 1719 1720]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu