https://eln.excellgen.com/index.php?action=history&feed=atom&title=1705_pT7A3-ZB2-T4Lysozyme 1705 pT7A3-ZB2-T4Lysozyme - Revision history 2026-10-18T04:06:38Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1705_pT7A3-ZB2-T4Lysozyme&diff=8997&oldid=prev Paul.xu at 16:18, 29 April 2013 2013-04-29T16:18:40Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1705 pT7A3-ZB2-T4Lysozyme&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1705 pT7A3-ZB2-T4Lysozyme <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-3- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1705&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1705 1706PCR.JPG]]&lt;br&gt; <br /> <br /> digest&amp;nbsp;1563 pT7A3-T4-Lysozyme and PCR products with Nco1 + Nde1&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 1705 1706.JPG]] <br /> <br /> ligate and transform&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-3- <br /> <br /> Make miniprep and digest with Nco1 + Nde1&lt;br&gt; <br /> <br /> [[Image:1705DIGEST.JPG‎ ]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&amp;amp;amp;amp;lt;br&amp;amp;amp;amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu