https://eln.excellgen.com/index.php?action=history&feed=atom&title=1708_2116_2115_2107Taq-muts 1708 2116 2115 2107Taq-muts - Revision history 2026-10-18T02:17:13Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1708_2116_2115_2107Taq-muts&diff=15622&oldid=prev Paul.xu at 22:08, 4 March 2017 2017-03-04T22:08:15Z <p></p> <p><b>New page</b></p><div>&lt;br/&gt;2014/3/<br /> <br /> [[File:1708 2116 2115 2107.jpg|RTENOTITLE]]<br /> <br /> <br /> <br /> {| width=&quot;200&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br/&gt;<br /> | colspan=&quot;3&quot; | 1&lt;br/&gt;<br /> | colspan=&quot;2&quot; | 2&lt;br/&gt;<br /> | 3&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 5&lt;br/&gt;<br /> | colspan=&quot;2&quot; | 6&lt;br/&gt;<br /> | 7&lt;br/&gt;<br /> | 8&lt;br/&gt;<br /> | 9&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 11&lt;br/&gt;<br /> | 12&lt;br/&gt;<br /> |-<br /> | clone ID<br /> | colspan=&quot;3&quot; | <br /> 1708<br /> <br /> pT7A3-HTNCre<br /> <br /> | colspan=&quot;2&quot; | <br /> 2116<br /> <br /> pT7A3-Penetratin-Cre<br /> <br /> | colspan=&quot;5&quot; | <br /> 2115<br /> <br /> pT7A3-iFLP<br /> <br /> | PageRuler Plus Prestained protein ladder&lt;br/&gt;<br /> | colspan=&quot;3&quot; | 2115<br /> pT7A3-iFLP<br /> <br /> | colspan=&quot;2&quot; | <br /> 2107<br /> <br /> taq-muts<br /> <br /> |-<br /> | host&lt;br/&gt;<br /> | colspan=&quot;5&quot; | &lt;br/&gt;Nico21DE3plysN&lt;br/&gt;<br /> | &lt;br/&gt;Nico21DE3plysN&lt;br/&gt;<br /> | &amp;nbsp; BL21(DE3)Star pRare2pLysS&amp;nbsp;&amp;nbsp;&amp;nbsp;&lt;br/&gt;<br /> | <br /> Nico21(DE3)<br /> <br /> pRare2pLysS<br /> <br /> | colspan=&quot;2&quot; | Tuner(de3)&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | colspan=&quot;5&quot; | Nico21DE3plysN&lt;br/&gt;<br /> |-<br /> | induction&lt;br/&gt;<br /> | colspan=&quot;5&quot; | 0.1 mM IPTG 25 oC o/n 5ml&lt;br/&gt;<br /> | colspan=&quot;5&quot; | 0.1mM IPTG 25 oC o/n&amp;nbsp; 5ml&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | colspan=&quot;5&quot; | N&lt;br/&gt;<br /> |-<br /> | purification&lt;br/&gt;<br /> | colspan=&quot;5&quot; | Ni beads&lt;br/&gt;<br /> | colspan=&quot;5&quot; | Ni beads&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | colspan=&quot;5&quot; | Ni column&lt;br/&gt;<br /> |-<br /> | MW&lt;br/&gt;<br /> | colspan=&quot;5&quot; | 10.30 / 42.8&lt;br/&gt;<br /> | colspan=&quot;5&quot; | pI/Mw: 9.76 / 52.8&lt;br/&gt;<br /> | 10,15, 25, 35, 55, 70, 100, 130, 250&lt;br/&gt;<br /> | colspan=&quot;3&quot; | 9.76 / 52.8&lt;br/&gt;<br /> | colspan=&quot;2&quot; | 5.67 / 92.8&lt;br/&gt;<br /> |}<br /> [[Category:Protein Purification]]</div> Paul.xu