https://eln.excellgen.com/index.php?action=history&feed=atom&title=1715_pT7A30-Pfu-sso7d 2026-10-18T18:30:25Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1715_pT7A30-Pfu-sso7d&diff=10489&oldid=prev 2013-10-25T19:54:12Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1715 pT7A30-Pfu-sso7d&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1715 pT7A30-Pfu-sso7d <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-10-21--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1715&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> subclone PFU from 367 to 1734 <br /> <br /> 367digest with NdeI+KpnI+DraIII+AlwnI(CUT OUT THE TOP BAND), 1734 digest with NdeI+KpnI <br /> <br /> [[Image:DIGEST_FOR1715_10_22_2013.JPG‎ ]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-10-23 <br /> <br /> Make miniprep, digest with NdeI+KpnI &lt;br&gt; <br /> <br /> [[Image:683_1715DIGEST.JPG‎ ]]<br /> <br /> STOCK 1715-4&lt;br&gt; <br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu