https://eln.excellgen.com/index.php?action=history&feed=atom&title=1726-pCMV5-hIgG-Lc-PKARIIb 1726-pCMV5-hIgG-Lc-PKARIIb - Revision history 2026-10-18T14:38:05Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1726-pCMV5-hIgG-Lc-PKARIIb&diff=8815&oldid=prev Paul.xu at 16:46, 8 April 2013 2013-04-08T16:46:32Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1726-pCMV5-hIgG-Lc-PKARIIb&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1519-pT7A3-T3-DNA-Ligase <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-4-5---2013-4-8 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1726&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1726 pcr 4-4-2013.JPG]]&lt;br&gt; <br /> <br /> [[Image:1726 clone 4-5-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-4-8 <br /> <br /> Make miniprep and digest with&amp;nbsp; Nhe1 + Kpn1&lt;br&gt; <br /> <br /> [[Image:1726 digest 4-8-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> Make miniprep and digest with BamH1&lt;br&gt; <br /> <br /> [[Image:1726_2digest_4-8-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {|<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> [[Category:Cloning]]</div> Paul.xu