https://eln.excellgen.com/index.php?action=history&feed=atom&title=1735_pT7A30-Exo-Taq-sso7d 1735 pT7A30-Exo-Taq-sso7d - Revision history 2026-10-18T16:06:36Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1735_pT7A30-Exo-Taq-sso7d&diff=9236&oldid=prev Paul.xu at 16:42, 23 May 2013 2013-05-23T16:42:42Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1735 pT7A30-Exo-Taq-sso7d&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1735 pT7A30-Exo-Taq-sso7d <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;May , 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1735&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> ligation using fragments from: &lt;br&gt; <br /> <br /> 1248 pT7A3-DeepVent-Exo, Nde1 + Nco1 ==&amp;gt; exo &lt;br&gt; <br /> <br /> 1249 pET-Taq_d289, Nco1 + Kpn1==&amp;gt; Taq <br /> <br /> &amp;nbsp; 368 pET30b-sso7d-KX, &amp;nbsp;&amp;nbsp;Kpn1+ Xho1==&amp;gt; sso7d &lt;br&gt; <br /> <br /> to Nde1 + Xho1 digested 1727 pT7A30 &lt;br&gt; <br /> <br /> [[Image:2DIGEST FOR 1735.JPG]][[Image:DIGEST FOR 1735.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony screen ===<br /> <br /> === Cloning ===<br /> <br /> ligation using fragments from: &lt;br&gt; <br /> <br /> 1248 pT7A3-DeepVent-Exo, Nde1 + Nco1 ==&amp;gt; exo <br /> <br /> 1249 pET-Taq_d289, Nco1 + Kpn1==&amp;gt; Taq <br /> <br /> to Nde1 + Kpn1 digested 1734&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 1747 1735.JPG]]&lt;br&gt; <br /> <br /> === Colony screen ===<br /> <br /> DIGEST WITH NdeI+NcoI+KpnI <br /> <br /> [[Image:1735digest.JPG]] <br /> <br /> [[Image:1735_4DIGEST2.JPG‎]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu