https://eln.excellgen.com/index.php?action=history&feed=atom&title=1737_2038Taq_2289 1737 2038Taq 2289 - Revision history 2026-10-18T11:23:47Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1737_2038Taq_2289&diff=12841&oldid=prev Excellgen at 14:47, 15 August 2014 2014-08-15T14:47:57Z <p></p> <p><b>New page</b></p><div>Aug11,2014&lt;br&gt; <br /> <br /> 1[[Image:1737 2289 1737.jpg]]&lt;br&gt; <br /> <br /> {| width=&quot;485&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Desc&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | MW, KDa&lt;br&gt; <br /> | purification&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;3&quot; | <br /> 1737 <br /> <br /> &lt;br&gt; <br /> <br /> | rowspan=&quot;3&quot; | <br /> &lt;br&gt; pT7A3E-His-TRX &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp;&amp;nbsp; &lt;br&gt; His-thioredoxin for making T7 DNA Polymerase complex <br /> <br /> | rowspan=&quot;3&quot; | &amp;nbsp;?mMIPTG, 25 oC o/n &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;3&quot; | Ni &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;3&quot; | 5.72/13.4 &lt;br&gt; <br /> | rowspan=&quot;3&quot; | <br /> &lt;br&gt; <br /> <br /> 8/8/2014 Manual run1&lt;br&gt; <br /> <br /> fraction4,5,6 <br /> <br /> |-<br /> | 2&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | pageRulerPlusPrestained protein ladder&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | rowspan=&quot;2&quot; | <br /> 1737 <br /> <br /> &lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | <br /> &lt;br&gt; pT7A3EHis-TRX&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> <br /> His-thioredoxin for making T7 DNA Polymerase complex &lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | &amp;nbsp;?mMIPTG, 25 oC o/n&lt;br&gt; <br /> | rowspan=&quot;2&quot; | <br /> Ni ------dialyse&lt;br&gt; <br /> <br /> ----<br /> &lt;br&gt; <br /> | rowspan=&quot;2&quot; | 5.72/13.4&lt;br&gt; <br /> | rowspan=&quot;2&quot; | 8/8/2014 Manual run1 <br /> fraction8,10&lt;br&gt; <br /> <br /> |-<br /> | 6&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | rowspan=&quot;3&quot; | 2289&lt;br&gt; <br /> | rowspan=&quot;3&quot; | <br /> pT7A30-SMT3 <br /> <br /> SUMO tag Saccharomyces cerevisiae S288c SUMO family protein SMT3 (SMT3), C-His tag&lt;br&gt; <br /> <br /> | rowspan=&quot;3&quot; | &amp;nbsp;?mMIPTG, 25 oC o/n&lt;br&gt; <br /> | rowspan=&quot;3&quot; | Ni ------dialyse <br /> ----<br /> Ni&lt;br&gt; <br /> | rowspan=&quot;3&quot; | 5.42/14.1&lt;br&gt; <br /> | rowspan=&quot;3&quot; | 8/8/2014 Manual run 9 <br /> fraction3,4,5&lt;br&gt; <br /> <br /> |-<br /> | 8&lt;br&gt;<br /> |-<br /> | 9&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | rowspan=&quot;3&quot; | <br /> 1737 &lt;br&gt; <br /> <br /> | rowspan=&quot;3&quot; | <br /> pT7A3EHis-TRX <br /> <br /> &lt;br&gt; His-thioredoxin for making T7 DNA Polymerase complex <br /> <br /> &lt;br&gt; <br /> <br /> | rowspan=&quot;3&quot; | &amp;nbsp;?mMIPTG, 25 oC o/n &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;3&quot; | Ni &lt;br&gt; &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;3&quot; | &lt;br&gt; 5.72/13.4&lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;3&quot; | 8/9/2014 Manual run 4 <br /> fraction3,4,5&lt;br&gt; <br /> <br /> |-<br /> | 11&lt;br&gt;<br /> |-<br /> | 12&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1737 2038taq.jpg]]&lt;br&gt; &lt;br&gt; <br /> <br /> {| width=&quot;485&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Desc&lt;br&gt; <br /> | Induction&lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | MW, KDa&lt;br&gt; <br /> | purification&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | 1737&lt;br&gt; <br /> | pT7A3EHis-TRX <br /> His-thioredoxin for making T7 DNA Polymerase complex &lt;br&gt; &lt;br&gt; <br /> <br /> | &amp;nbsp;?mMIPTG, 25 oC o/n &lt;br&gt; &lt;br&gt; <br /> | Ni &lt;br&gt; &lt;br&gt; <br /> | &amp;nbsp; 5.72/13.4 &lt;br&gt; &lt;br&gt; <br /> | <br /> &lt;br&gt; 8/9/2014 &lt;br&gt; <br /> <br /> Manual run 4 <br /> <br /> &lt;br&gt; <br /> <br /> fraction6 <br /> <br /> &lt;br&gt; <br /> <br /> |-<br /> | 2&lt;br&gt; <br /> | rowspan=&quot;4&quot; | 2038 &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;4&quot; | L42-sso7d-KlenTaq&lt;br&gt; <br /> | rowspan=&quot;4&quot; | &amp;nbsp;?mMIPTG, 25 oC o/n &lt;br&gt; <br /> | rowspan=&quot;4&quot; | Ni &lt;br&gt; <br /> | rowspan=&quot;4&quot; | 7.02/70.1&lt;br&gt; <br /> | rowspan=&quot;4&quot; | <br /> 8/11/2014 &lt;br&gt; <br /> <br /> Manual run0 <br /> <br /> fraction9,10,11,12&lt;br&gt; <br /> <br /> |-<br /> | 3&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | <br /> pageRulerPlusPrestained protein ladder &lt;br&gt; <br /> <br /> | &lt;br&gt; &lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> | &lt;br&gt; &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | rowspan=&quot;6&quot; | 2038&lt;br&gt;&lt;br&gt; <br /> | rowspan=&quot;6&quot; | L42-sso7d-KlenTaq&lt;br&gt; <br /> | rowspan=&quot;6&quot; | &amp;nbsp;?mMIPTG, 25 oC o/n &lt;br&gt; <br /> | rowspan=&quot;6&quot; | Ni &lt;br&gt; <br /> | rowspan=&quot;6&quot; | 7.02/70.1&lt;br&gt; <br /> | rowspan=&quot;6&quot; | 8/11/2014 <br /> Manual run0 <br /> <br /> fraction13&lt;br&gt; <br /> <br /> 8/10/2014 <br /> <br /> Manual run7 <br /> <br /> fraction11,12,13,14,19&lt;br&gt; <br /> <br /> |-<br /> | 8&lt;br&gt;<br /> |-<br /> | 9&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt;<br /> |-<br /> | 11&lt;br&gt;<br /> |-<br /> | 12&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Purification]]</div> Excellgen