https://eln.excellgen.com/index.php?action=history&feed=atom&title=1738_pEC-iMMLV-m 1738 pEC-iMMLV-m - Revision history 2026-10-18T18:54:24Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1738_pEC-iMMLV-m&diff=8942&oldid=prev Paul.xu at 18:39, 19 April 2013 2013-04-19T18:39:59Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1738 pEC-iMMLV-m&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1738 pEC-iMMLV-m <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-4- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1738&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> Date:2013-3-&lt;br&gt; <br /> <br /> PCR use 1714 as template and primers &lt;br&gt; <br /> <br /> MMLV-EcoR1-Nde1-F + MMLV-W313F-R &lt;br&gt; <br /> <br /> MMLV-W313F-F + MMLV-L435G-R &lt;br&gt; <br /> <br /> MMLV-L435G-F + MMLV-Not1-Xho1-R &lt;br&gt; <br /> <br /> [[Image:1738 1-2-3PCR.JPG]]&lt;br&gt; <br /> <br /> join 3 fragments by pcr using&lt;br&gt; <br /> <br /> MMLV-EcoR1-Nde1-F atcgac gaattc CAT ATG ACCCTAAATATAGAAGATGAGC <br /> <br /> MMLV-Not1-Xho1-R (CTCCAGACACCTCTACCCTCCTC cat cac cat cac cat cac taa GCGGCCGC CTCGAG cgatca) tgatcgCTCGAGGCGGCCGCttagtgatggtgatggtgatgGAGGAGGGTAGAGGTGTCTGGAG <br /> <br /> [[Image:1738PCR.JPG]]&lt;br&gt; <br /> <br /> digest with&amp;nbsp; EcoR1 + Xho1 <br /> <br /> [[Image:DIGEST FOR 1738.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013 <br /> <br /> DIGEST WITH BamHI(ONLY ONE BAND)&lt;br&gt; <br /> <br /> [[Image:1738digest_with_BamhI.JPG‎]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu