https://eln.excellgen.com/index.php?action=history&feed=atom&title=1739-pT7A3-ZB2-wtSM2 1739-pT7A3-ZB2-wtSM2 - Revision history 2026-10-18T15:41:50Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1739-pT7A3-ZB2-wtSM2&diff=9774&oldid=prev Paul.xu at 20:06, 18 July 2013 2013-07-18T20:06:45Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1739-pT7A3-ZB2-wtSM2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1739-pT7A3-ZB2-wtSM2<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-5-6---2013-5-9 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1739&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1619+1731+1739+1742+1743+1744 pcr 4-26-2013.JPG]] <br /> <br /> [[Image:1619+1739+1742+1748 clone 5-7-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-5-9 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1739 digest 5-9-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {| cellspacing=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1739-pT7A3-ZB2-wtSM2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1739-pT7A3-ZB2-wtSM2<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-7-15---2013-7-18 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1739&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:651+1035+1739PCR_7-12-2013.jpg]]&lt;br&gt; <br /> <br /> PCR wtSM2 using&lt;br&gt;<br /> <br /> template is 1680pCold-GST-wtSM2&lt;br&gt;WtSM2-1 &lt;br&gt;WtSM2-Xho1-R&lt;br&gt;<br /> <br /> [[Image:1739clone_7-16-2013.jpg]]&lt;br&gt;<br /> <br /> {| width=&quot;387&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; height=&quot;81&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | 1753,pT7A3-ZB2-Keap1&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Xho1 + Nde1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-7-18 <br /> <br /> Make miniprep and digest with Nde1 + Xho1 <br /> <br /> [[Image:1739digest_7-18-2013.JPG]]&lt;br&gt; <br /> <br /> clone5 for sequencing<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {| cellspacing=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu