https://eln.excellgen.com/index.php?action=history&feed=atom&title=1746_pT7A3-hINFA2 1746 pT7A3-hINFA2 - Revision history 2026-10-18T08:19:15Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1746_pT7A3-hINFA2&diff=8970&oldid=prev Paul.xu: Created page with "== '''Cloning of 1746 pT7A3-hINFA2''' == '''Purpose:''' Cloning of 1746 pT7A3-hINFA2 '''Date: '''2013-4--- '''Notebook:0''' '''Page:''' '''Clone ID:1746''' '''Experim..." 2013-04-25T19:35:15Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1746 pT7A3-hINFA2&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1746 pT7A3-hINFA2 &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-4--- &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:1746&#039;&#039;&#039; &#039;&#039;&#039;Experim...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1746 pT7A3-hINFA2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1746 pT7A3-hINFA2 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-4--- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1746&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === subcloning ===<br /> <br /> subclone from 11711 to 1279 pT7A3 using Nco1 + Xho1 <br /> <br /> [[Image:DIGEST_FOR1746_1265FIX_04_23_2013.JPG‎ ]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 281px; height: 66px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 3&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 1711&lt;br&gt; <br /> | 1279pT7A3&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;Nco1 + Xho1 &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-2- <br /> <br /> Make miniprep and digest with Nco1 + Xho1 <br /> <br /> [[Image:1746digest_04_25_2013.JPG‎ ]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==</div> Paul.xu