https://eln.excellgen.com/index.php?action=history&feed=atom&title=1747_pT7A30-iMMLV 1747 pT7A30-iMMLV - Revision history 2026-10-18T15:40:35Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1747_pT7A30-iMMLV&diff=9223&oldid=prev Paul.xu at 20:20, 22 May 2013 2013-05-22T20:20:24Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1747 pT7A30-iMMLV&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1747 pT7A30-iMMLV <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;May , 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1747&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> subclone to 1727 pT7A30 using Nde1 + Xho1 <br /> <br /> digest 1738 with Nde1 + Xho1+DraIII+AlwN1,should get four bands(==&amp;gt;2000bp,==&amp;gt;1600bp,==&amp;gt;800bp,==&amp;gt;500bp),the 2000bp DNA is iMMLV&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 1734 1747.JPG]]&lt;br&gt; <br /> <br /> === Colony screen ===<br /> <br /> === Cloning ===<br /> <br /> subclone to 1727 pT7A30 using Nde1 + Xho1&lt;br&gt; <br /> <br /> digest 1738 with Nde1 + Xho1+BamHI,should get 3 bands,cut out the lower two bands. <br /> <br /> [[Image:DIGEST FOR 1747 1735.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony screen ===<br /> <br /> [[Image:1747digest.JPG‎ ]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu