https://eln.excellgen.com/index.php?action=history&feed=atom&title=1750_pT7A3-Tks 1750 pT7A3-Tks - Revision history 2026-10-18T16:31:22Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1750_pT7A3-Tks&diff=9348&oldid=prev Paul.xu at 21:33, 3 June 2013 2013-06-03T21:33:22Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1750 pT7A3-Tks&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1750 pT7A3-Tks <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;May , 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1750&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> subclone from 1265 to 1279 <br /> <br /> 1279 digest with NdeI +XhoI&lt;br&gt; <br /> <br /> 1265 digest with Nde1 + Xho1(should get three bands, because there is one XhoI site in TKS, cut out the lower two bands)&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 1750 WITH NdeI+XhoI.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> digest with Nde1 + Xho1&amp;nbsp; <br /> <br /> [[Image:1750digest.JPG]] <br /> <br /> DIGEST_WITH_HindIII ,SHOULD GET ONE BAND&lt;br&gt; <br /> <br /> [[Image:1750 4DIGEST WITH HindIII.JPG]] <br /> <br /> DIGEST WITH NdeI+KpnI<br /> <br /> [[Image:1750DIGESTWITHNNdeI+KpnI.JPG]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu