https://eln.excellgen.com/index.php?action=history&feed=atom&title=1751-pBad24-SOC-_HOC 1751-pBad24-SOC- HOC - Revision history 2026-10-18T19:41:16Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1751-pBad24-SOC-_HOC&diff=9470&oldid=prev Paul.xu at 22:39, 13 June 2013 2013-06-13T22:39:14Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1751-pBad24-SOC- HOC&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1751-pBad24-SOC- HOC <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-6-11---2013-6-13 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1751&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1766 digest 6-11-2013.JPG]]&lt;br&gt; <br /> <br /> insert: 1766 pUC57-SOC-HOC digest with EcoR1+Hind111+Bsa1 at 37C&lt;br&gt; <br /> <br /> [[Image:1751clone 6-11-2013.JPG]]&lt;br&gt; <br /> <br /> band 4: vector: 546 pBad24-sfGFP digest with EcoR1+Hind111&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === &amp;nbsp;Colony Screening ===<br /> <br /> Date: 2013-6-13 <br /> <br /> Make miniprep and digest with EcoR1+Hind111 <br /> <br /> [[Image:1751 digest 6-13-2013.JPG]][[Image:1751digest1 6-13-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> digest with Xba1+Hind111 &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1751-pBad24-SOC- HOC&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1751-pBad24-SOC- HOC <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-6-12---2013-6-13 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1751&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1751clone 6-12-2013.JPG]]&lt;br&gt; <br /> <br /> vector: 546 pBad24-sfGFP digest with EcoR1+Hind111&lt;br&gt; <br /> <br /> [[Image:1766 1st digest 6-12-2013.JPG]]&lt;br&gt; <br /> <br /> 1766 pUC57-SOC-HOC digest with EcoR1+Hind111, cut the band and gel extraction ,then digest with Bsa1 again at 50C&lt;br&gt; <br /> <br /> run gel &lt;br&gt; <br /> <br /> [[Image:1766 2nd diget6-12-2013.JPG]]&lt;br&gt; <br /> <br /> &amp;nbsp;cut large band and small band seperately, and do ligation with vector seperately&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-6-13 <br /> <br /> Make miniprep and digest with EcoR1+Hind111 <br /> <br /> large band ligation digestion result: <br /> <br /> [[Image:1751 Ldigest 6-13-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> large band ligation digestion result: EcoR1+Hind111 ,&amp;nbsp; Xba1+Hind111 &lt;br&gt; <br /> <br /> [[Image:1751_digest_3_6-13-2013.JPG]]&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> small band ligation digestion result: <br /> <br /> [[Image:1751 Sdigest 6-13-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu