https://eln.excellgen.com/index.php?action=history&feed=atom&title=1756_pCold-6H-%28TEV%29-MBP-%283C%29 1756 pCold-6H-(TEV)-MBP-(3C) - Revision history 2026-10-18T21:18:18Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1756_pCold-6H-(TEV)-MBP-(3C)&diff=9431&oldid=prev Paul.xu at 02:28, 12 June 2013 2013-06-12T02:28:36Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1756 pCold-6H-(TEV)-MBP-(3C)&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1756 pCold-6H-(TEV)-MBP-(3C) <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;June 10, 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook: ???&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page: ???&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1756&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> subclone His-TEV-MBP from 1717 pT7A3-6H-(TEV)-MBP-(3C) using Nco1 + Nde1 TO 1755 <br /> <br /> [[Image:DIGEST FOR 1756 1762.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> digest with Nco1 + Nde1&amp;nbsp; <br /> <br /> [[Image:1756_screening_20130511.JPG]]&lt;br&gt; <br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Paul.xu