https://eln.excellgen.com/index.php?action=history&feed=atom&title=1758_1762_1779_1759 1758 1762 1779 1759 - Revision history 2026-10-18T05:50:41Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1758_1762_1779_1759&diff=9845&oldid=prev Paul.xu at 16:28, 26 July 2013 2013-07-26T16:28:58Z <p></p> <p><b>New page</b></p><div>== Protein Purification ==<br /> <br /> 2013/7/16&lt;br&gt; SHANYI <br /> <br /> [[Image:1758 1726 1779 1759.jpg]] <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;982&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | &lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | pI/Mw&lt;br&gt; <br /> | Expression &lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;2&quot; | <br /> pCMV5-hIgG-Lc-FN3K &lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | FN3K&lt;br&gt; <br /> | rowspan=&quot;2&quot; | <br /> 1758&lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | 293EF&lt;br&gt; <br /> | &lt;br&gt; <br /> | rowspan=&quot;2&quot; | Histrap excel&lt;br&gt; <br /> | rowspan=&quot;2&quot; | 7.15 / 62756.42&lt;br&gt; <br /> | rowspan=&quot;2&quot; | <br /> no&amp;nbsp; <br /> <br /> |-<br /> | 2&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &amp;nbsp; pCMV5-hIgG-Lc-PKARIIb&lt;br&gt; <br /> | rowspan=&quot;2&quot; | PKARIIb&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &amp;nbsp; 1726&lt;br&gt; <br /> | rowspan=&quot;2&quot; | 293EF&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &lt;br&gt; &lt;br&gt; <br /> | rowspan=&quot;2&quot; | Histrap excel&lt;br&gt; <br /> | rowspan=&quot;2&quot; | 5.52 / 75918.93&lt;br&gt; <br /> | rowspan=&quot;2&quot; | &amp;nbsp;no&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | <br /> PageRuler Plus Prestained protein ladder <br /> <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | pTCold-MBP-(3C-TEV)-NRF2&lt;br&gt; <br /> | NRF2&lt;br&gt; <br /> | 1779&lt;br&gt; <br /> | <br /> Nico21(DE3)pRare2pLysS&lt;br&gt; <br /> <br /> | <br /> NRF20 <br /> <br /> 0.36mg/ml&lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | <br /> Ni column &lt;br&gt; <br /> <br /> Amylose column&lt;br&gt; <br /> <br /> Histrap&lt;br&gt; <br /> <br /> Amylose beads&lt;br&gt; <br /> <br /> Flowthrough&lt;br&gt; <br /> <br /> | <br /> 4.67 / 68345.09 &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | pTCold-MBP-(3C-TEV)-NRF2&lt;br&gt; <br /> | NRF2&lt;br&gt; <br /> | 1779&lt;br&gt; <br /> | &amp;nbsp;Nico21(DE3)pRare2pLysS&lt;br&gt; <br /> | NRF20 <br /> 1mg/ml&lt;br&gt; <br /> <br /> | <br /> 4.67 / 68345.09 &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | pTCold-MBP-(3C-TEV)-NRF2&lt;br&gt; <br /> | &lt;br&gt; <br /> | 1779&lt;br&gt; <br /> | &amp;nbsp; Nico21(DE3)pRare2pLysS&lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> | <br /> After TEVdigestion <br /> <br /> elute from Histrap&lt;br&gt; <br /> <br /> | <br /> &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | pTCold-MBP-(3C-TEV)-NRF2&lt;br&gt; <br /> | &lt;br&gt; <br /> | 1779&lt;br&gt; <br /> | &amp;nbsp; Nico21(DE3)pRare2pLysS&lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> | After TEVdigestion <br /> elute from Amylose beads&lt;br&gt; <br /> <br /> | &amp;nbsp; &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | &lt;br&gt; <br /> | keapI&lt;br&gt; <br /> | &lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | &lt;br&gt; <br /> | keapI&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt;&lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu