https://eln.excellgen.com/index.php?action=history&feed=atom&title=1759_pCold-6H-%28TEV%29-MBP-%283C%29-Keap1 1759 pCold-6H-(TEV)-MBP-(3C)-Keap1 - Revision history 2026-10-18T18:59:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1759_pCold-6H-(TEV)-MBP-(3C)-Keap1&diff=9740&oldid=prev Paul.xu at 17:27, 17 July 2013 2013-07-17T17:27:03Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1759 pCold-6H-(TEV)-MBP-(3C)-Keap1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of Keap1 into pCold-6H-(TEV)-MBP-(3C) <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;June 11, 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;???&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&amp;nbsp;???&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1759&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> subclone KEAP1 from 1676 pCold-MBP-(3C)-KEAP1 to 1756 pCold-6H-(TEV)-MBP-(3C) using Nde1 + Xho1 &lt;br&gt; <br /> <br /> [[Image:Digest for1759.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> digest with&amp;nbsp; Nde1 +Xho1 <br /> <br /> [[Image:1759digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> === &#039;&#039;&#039;protein expression&#039;&#039;&#039;&lt;br&gt; ===<br /> <br /> [[Image:Nrf2+cre+keap protein 7-15-2013.jpg]]&lt;br&gt; <br /> <br /> {| width=&quot;362&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | clone &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1 to 8&lt;br&gt; <br /> | NRF2&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | Cre&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | 1759,pCold-6H-(TEV)-MBP-(3C)-Keap1&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | 1759,pCold-6H-(TEV)-MBP-(3C)-Keap1&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu