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1768-pET28b-6H-(Tev)-Tat-HA - Revision history
2026-10-18T17:28:24Z
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Paul.xu at 19:25, 17 July 2013
2013-07-17T19:25:53Z
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<p><b>New page</b></p><div>== '''Cloning of 1768-pET28b-6H-(Tev)-Tat-HA''' ==<br />
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'''Purpose:''' Cloning of 1768-pET28b-6H-(Tev)-Tat-HA <br />
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'''Date: '''2013-7-12---2013-7-17 <br />
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'''Notebook:0''' <br />
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'''Page:''' <br />
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'''Clone ID:1768''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:1768PCR 6-24-2013.JPG]]<br> <br />
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gene synthesis<br> <br />
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Nco1-6H-Tev-Tat-HA-Nde1-1 to Nco1-6H-Tev-Tat-HA-Nde1-4<br> <br />
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[[Image:651+1768clone 7-15-2013.jpg]]<br> <br />
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{| width="237" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| lane<br> <br />
| 3<br> <br />
| 4<br><br />
|-<br />
| <br> <br />
| PCR product<br> <br />
| pET28b<br><br />
|-<br />
| enzymes<br> <br />
| colspan="2" | &nbsp;&nbsp;&nbsp; Nco1 + Nde1<br><br />
|}<br />
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=== Colony Screening ===<br />
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Date: 2013-7-17<br />
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Make miniprep and digest with Nco1 + Nde1<br />
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[[Image:651+1768_digest_7-17-2013.jpg]]<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu