https://eln.excellgen.com/index.php?action=history&feed=atom&title=1769-pET28b-6His-NRF2 1769-pET28b-6His-NRF2 - Revision history 2026-10-18T19:26:55Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1769-pET28b-6His-NRF2&diff=10022&oldid=prev Paul.xu at 15:28, 13 August 2013 2013-08-13T15:28:58Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1769-pET28b-6His-NRF2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1769-pET28b-6His-NRF2 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-6-17--2013-6-18 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039;&amp;nbsp; 006026 <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;&amp;nbsp;&amp;nbsp; 006189-006190 <br /> <br /> &#039;&#039;&#039;Clone ID:1769&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1769clone 6-17-2013.JPG]] <br /> <br /> subclone from 1:1661 pT7A3-MBP-(3c)-NRF2&amp;nbsp; to 2:559pET28b-Nhe1 <br /> <br /> using Nde1 + Xho1&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-6-18 <br /> <br /> Make miniprep and digest with&amp;nbsp; Nde1 + Xho1 <br /> <br /> [[Image:1769_digest_6-18-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> == protein expression result of NRF2 clones &lt;br&gt; ==<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;569&quot;<br /> |-<br /> | clone ID&lt;br&gt; <br /> | expression&lt;br&gt; <br /> | gel reslut&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1626, pET28-cold-NRF2&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1625 1626 1656]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1661,pT7A3-MBP-(3c)-NRF2&lt;br&gt; <br /> | yes,&lt;br&gt; <br /> | [[1661 1674 1683]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1674,pCold-GST-(3c)-NRF2&lt;br&gt; <br /> | need to use GST beads&lt;br&gt; <br /> | [[1661 1674 1683]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1682,pCold-6His-GST-(E)-(3C)-NRF2&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | [[1661 1682 1683 1719 1748 1752 1771 1779]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1683,pCold-MBP-(3C)-NRF2&lt;br&gt; <br /> | yes, &lt;br&gt; <br /> | [[1661 1674 1683]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1698,pT7A3-NRF2&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1676 1759 1698 1769 1747 1769 1757 1682p]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1719,pT7A3-MBP-(E3C)-NRF2&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | [[631 1497 1542 1719 1748 1765 1780 1781 1782 1797]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1748,pCold-8His-MBP-NRF2-6His&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | [[631 1497 1542 1719 1748 1765 1780 1781 1782 1797]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1752,pCold-TF-(E3C)-NRF2&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | [[1661 1682 1683 1719 1748 1752 1771 1779]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1769,pET28b-6His-NRF2&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1676 1759 1698 1769 1747 1769 1757 1682p]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1796,pT7A3-MBP-(3C-TEV)-NRF2-6His&lt;br&gt; <br /> | not sure&lt;br&gt; <br /> | [[1720 1757 1759 1796 1800 1803 1804 1809 1759 MBP-Keap1 1719]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1779,pTCold-MBP-(3C-TEV)-NRF2&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | [[1661 1682 1683 1719 1748 1752 1771 1779]]&lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt;<br /> [[Category:Cloning]]</div> Paul.xu