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1770 pT7A3-CBD - Revision history
2026-10-18T14:54:21Z
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Paul.xu at 20:44, 24 July 2013
2013-07-24T20:44:43Z
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<p><b>New page</b></p><div>== '''Cloning of 1770 pT7A3-CBD''' ==<br />
<br />
'''Purpose:''' Cloning of 1770 pT7A3-CBD <br />
<br />
'''Date: '''2013-7--1- <br />
<br />
'''Notebook:05846''' <br />
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'''Page:''' <br />
<br />
'''Clone ID:1770''' <br />
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'''Experimenters:''' Shanyi <br />
<br />
== '''Results''' ==<br />
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=== cloning ===<br />
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pcr <br> <br />
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[[Image:654 655 1770pcr.JPG]]<br> <br />
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digest with Xba1+ Nde1 <br> <br />
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[[Image:Digest for654 655 1770.JPG]] <br />
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<br> <br />
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=== Colony Screening ===<br />
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Date: 2013-7-2 <br />
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Make miniprep and digest with Xba1+ Nde1 <br />
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[[Image:654 655 1770DIGEST.JPG]] <br />
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SEND 1770-1 TO SEQUENCE, due to the wrong oligo primer, the clone missing one g<br> <br />
<br />
== '''Cloning of 1770 pT7A3-CBD''' ==<br />
<br />
'''Purpose:''' Cloning of 1770 pT7A3-CBD <br />
<br />
'''Date: '''2013-7--1- <br />
<br />
'''Notebook:05846''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:1770''' <br />
<br />
'''Experimenters:''' Shanyi <br />
<br />
== '''Results''' ==<br />
<br />
=== cloning ===<br />
<br />
pcr (use 1770-1 as template)<br> <br />
<br />
[[Image:1770fixpcr.JPG]]<br> <br />
<br />
digest with Xba1+ Nde1 <br> <br />
<br />
[[Image:Digestfor1770fix.JPG]]<br> <br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2013-7-2 <br />
<br />
Make miniprep and digest with Xba1+ Nde1 <br />
<br />
[[Image:1770FIXDIGEST2.JPG ]]<br> <br />
<br />
<br> <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
== '''Conclusion''' ==<br />
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[[Category:Cloning]]</div>
Paul.xu