https://eln.excellgen.com/index.php?action=history&feed=atom&title=1771-pT7A3-MBP-%283C%29-PCK1 1771-pT7A3-MBP-(3C)-PCK1 - Revision history 2026-10-18T14:45:26Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1771-pT7A3-MBP-(3C)-PCK1&diff=9707&oldid=prev Paul.xu: Created page with "== '''Cloning of 1771-pT7A3-MBP-(3C)-FN3K''' == '''Purpose:''' Cloning of 1771-pT7A3-MBP-(3C)-FN3K '''Date: '''2013-6-6---2013-6-7 '''Notebook:0''' '''Page:''' '''Clone..." 2013-07-12T15:04:41Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1771-pT7A3-MBP-(3C)-FN3K&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1771-pT7A3-MBP-(3C)-FN3K &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-6-6---2013-6-7 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1771-pT7A3-MBP-(3C)-FN3K&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1771-pT7A3-MBP-(3C)-FN3K <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-6-6---2013-6-7 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1771&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1682+1757clone 6-6-2013.JPG]]&amp;lt;br&amp;gt; <br /> <br /> subclone from &amp;lt;br&amp;gt;Harvard Kinase Plate HRG007900, 79&amp;lt;br&amp;gt;Well: G02&amp;lt;br&amp;gt;3,donor vector: pDNR-DUAL&amp;lt;br&amp;gt;4,acceptor vector: 1565 pT7A3-MBP-(3C)-sfGFP2&amp;lt;br&amp;gt;Enzyme: Sal1 + HindIII <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-6-7 <br /> <br /> Make miniprep and digest with Sal1 + HindIII <br /> <br /> [[Image:1757 digest 6-7-2013.JPG]]&amp;lt;br&amp;gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> 2013-6-12&amp;lt;br&amp;gt; <br /> <br /> [[Image:652+1757 digest 6-12-2013.JPG]] <br /> <br /> 652 pT7A30-hIFNA2 and 1757pT7A3-MBP-(3C)-FN3K digest with Sal1+Hind111&amp;lt;br&amp;gt; <br /> <br /> [[Image:1757-2 digest 6-12-2013.JPG]] <br /> <br /> 1757pT7A3-MBP-(3C)-FN3K digest with AlwN1&amp;lt;br&amp;gt; <br /> <br /> Sequence result show:&amp;amp;nbsp; this is PCK1&amp;amp;nbsp; Plate: HRG007900, well: G02 ,&amp;lt;br&amp;gt; <br /> <br /> clone 1771 <br /> <br /> clone 1757 should subclone again &lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu