https://eln.excellgen.com/index.php?action=history&feed=atom&title=1773_pUltraBac-NRF2 2026-10-18T15:05:35Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1773_pUltraBac-NRF2&diff=9653&oldid=prev 2013-07-10T16:30:21Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of1773 pUltraBac-NRF2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1773 pUltraBac-NRF2 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-7--2- <br /> <br /> &#039;&#039;&#039;Notebook:05846&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1773&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> PCR using 1622 pLX304-NRF2 as template&lt;br&gt; <br /> <br /> [[Image:1773pcr.JPG]]http://www.excellgen.net/eln/images/1/16/1773pcr.JPG&lt;br&gt; <br /> <br /> digest PCR products with BamH1 + Xba1, then ligate to BamH1 + Xba1 digested 1246 pUltrabac&lt;br&gt; <br /> <br /> [[Image:Digest for 1773 1775 1662.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-7- <br /> <br /> Make miniprep and digest with Xho1 + BamH1 <br /> <br /> [[Image:1662 1773 1775 1776 1777 1778digest.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu