https://eln.excellgen.com/index.php?action=history&feed=atom&title=1781-pTCold-MBP-%283C-TEV%29-PKARIIb 1781-pTCold-MBP-(3C-TEV)-PKARIIb - Revision history 2026-10-18T11:28:23Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1781-pTCold-MBP-(3C-TEV)-PKARIIb&diff=9784&oldid=prev Paul.xu at 17:39, 19 July 2013 2013-07-19T17:39:59Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1781-pTCold-MBP-(3C-TEV)-PKARIIb&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1781-pTCold-MBP-(3C-TEV)-PKARIIb <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-7-17---2013-7-19<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1781&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1781PCR 7-16-2013.JPG]]&lt;br&gt; <br /> <br /> PCR using 1728 as template&lt;br&gt;ratPKARIIb-Nhe1-F&lt;br&gt;PKARIIb-Xho1-R&lt;br&gt; <br /> <br /> [[Image:556+1781+1783clone 7-17-2013.jpg]]&lt;br&gt; <br /> <br /> {| width=&quot;356&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1677, pTCold-MBP-(3C)&lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp;&amp;nbsp; Nhe1+Xho1&lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-7-19<br /> <br /> Make miniprep and digest Nhe1+Xho1&lt;br&gt; <br /> <br /> [[Image:1781+1783_digest_7-19-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu