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2026-10-18T15:05:37Z
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Paul.xu: Created page with "== '''Cloning of 1783-pT7A31''' == '''Purpose:''' Cloning of 1783-pT7A31 '''Date: '''2013-7-17---2013-7-19 '''Notebook:006026''' '''Page:''' '''Clone ID:1783''' '''Exp..."
2013-07-19T17:44:22Z
<p>Created page with "== '''Cloning of 1783-pT7A31''' == '''Purpose:''' Cloning of 1783-pT7A31 '''Date: '''2013-7-17---2013-7-19 '''Notebook:006026''' '''Page:''' '''Clone ID:1783''' '''Exp..."</p>
<p><b>New page</b></p><div>== '''Cloning of 1783-pT7A31''' ==<br />
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'''Purpose:''' Cloning of 1783-pT7A31<br />
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'''Date: '''2013-7-17---2013-7-19 <br />
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'''Notebook:006026''' <br />
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'''Page:''' <br />
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'''Clone ID:1783''' <br />
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'''Experimenters:''' yingying <br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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[[Image:556+1781+1783clone_7-17-2013.jpg]]<br><br />
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{| width="307" cellspacing="1" cellpadding="1" border="1" height="81"<br />
|-<br />
| lane<br><br />
| 5<br><br />
|-<br />
| <br><br />
| 651,pT7A30-IFN-gamma<br><br />
|-<br />
| enzymes<br><br />
| Nde1+Xho1<br><br />
|}<br />
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digest vector with Nde1 + Xho1,<br>Ligate to annealed oligos<br>Nde1-Sal1-T+Nde1-Sal1-B<br><br />
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=== Colony Screening ===<br />
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Date: 2013-7-19<br />
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Make miniprep and digest with HindIII + Sma1, should get one band (lineared, HindIII is gone)<br><br />
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[[Image:1781+1783_digest_7-19-2013.JPG]]<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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<br> [[Category:Cloning]]</div>
Paul.xu