https://eln.excellgen.com/index.php?action=history&feed=atom&title=1789_pT7A31-TEV-%283c%29-ELP 2026-10-18T14:43:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1789_pT7A31-TEV-(3c)-ELP&diff=9854&oldid=prev 2013-07-26T16:55:37Z

<p>Created page with &quot;== &#039;&#039;&#039;Cloning of 1789 pT7A31-TEV-(3c)-ELP&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1789 pT7A31-TEV-(3c)-ELP &#039;&#039;&#039;Date: &#039;&#039;&#039;07,26, 2013&lt;br&gt; &#039;&#039;&#039;Notebook: &#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clon...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1789 pT7A31-TEV-(3c)-ELP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1789 pT7A31-TEV-(3c)-ELP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;07,26, 2013&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1789&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;Shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR from 432 pMBP-7xHis-TEV using these primers:TEV-Nco1-F and TEV-(3C)-Sal1-R <br /> <br /> [[Image:1788_1789PCR.JPG]]<br /> <br /> digest vector: Nco1 + Xho1 PCR: Nco1 + Sal1 <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> digest with <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Paul.xu