https://eln.excellgen.com/index.php?action=history&feed=atom&title=1792-pT7A3-TthPol 1792-pT7A3-TthPol - Revision history 2026-10-18T10:10:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1792-pT7A3-TthPol&diff=10049&oldid=prev Paul.xu at 18:41, 15 August 2013 2013-08-15T18:41:01Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1792-pT7A3-TthPol&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1792-pT7A3-TthPol <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-6--2013-8-8 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1792&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1792+1793clone 8-6-2013.JPG]] <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;336&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 483,pET28b-TthPol &lt;br&gt; <br /> | 1279, pT7A3&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 + EcoR1&lt;br&gt;<br /> |}<br /> <br /> === &lt;br&gt;Colony Screening &lt;br&gt; ===<br /> <br /> Date: 2013-8-9 <br /> <br /> Make miniprep and digest with Nde1 + EcoR1&lt;br&gt; <br /> <br /> [[Image:1792 digest 8-9-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of 1792-pT7A3-TthPol&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1792-pT7A3-TthPol <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-13--2013-8-15 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1792&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1792clone_8-13-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;336&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 483,pET28b-TthPol &lt;br&gt; <br /> | 1279, pT7A3&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | Nde1 + EcoR1&lt;br&gt;<br /> |}<br /> <br /> === &lt;br&gt;Colony Screening &lt;br&gt; ===<br /> <br /> Date: 2013-8-15 <br /> <br /> Make miniprep and digest with Nde1 + EcoR1&lt;br&gt; <br /> <br /> [[Image:1792diges_8-15-2013.JPG]]&lt;br&gt; <br /> <br /> digest with Nde1 + BamH1&lt;br&gt;<br /> <br /> [[Image:1792digest2_8-15-2013.JPG]]<br /> <br /> clone 1 for stock<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu