https://eln.excellgen.com/index.php?action=history&feed=atom&title=1793-pET28b-VPol1 1793-pET28b-VPol1 - Revision history 2026-10-18T05:50:57Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1793-pET28b-VPol1&diff=10254&oldid=prev Paul.xu: moved 1793-pEC-VPol1 to 1793-pET28b-VPol1 2013-09-13T20:05:33Z <p>moved <a href="/index.php?title=1793-pEC-VPol1" class="mw-redirect" title="1793-pEC-VPol1">1793-pEC-VPol1</a> to <a href="/index.php?title=1793-pET28b-VPol1" title="1793-pET28b-VPol1">1793-pET28b-VPol1</a></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1793-pET28b-VPol1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1793-pET28b-VPol1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-1----2013-8-7 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1793&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1793pcr+1807+1808PCR 8-2-2013.JPG]][[Image:1793pcr2 8-2-2013.JPG]]&lt;br&gt; <br /> <br /> gene synthesis&lt;br&gt; <br /> <br /> VPol1-1-1&amp;nbsp; to VPol1-1-18&lt;br&gt; <br /> <br /> VPol1-2-1 to VPol1-1-2-14&lt;br&gt; <br /> <br /> VPol1-3-1 to VPol1-3-16&lt;br&gt; <br /> <br /> [[Image:1793PCR 8-5-2013.JPG]]&lt;br&gt; <br /> <br /> template:pcr1+pcr2+pcr3&lt;br&gt; <br /> <br /> VPol1-1-1+VPol1-3-16&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1792+1793clone 8-6-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;394&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1074,pET28b-IHFab&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | Nco1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-7 <br /> <br /> Make miniprep and digest with Nco1 + Xho1 <br /> <br /> [[Image:1793digest 8-7-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Fixing of Cloning of 1793-pET28b-VPol1&#039;&#039;&#039;&lt;br&gt; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; fixing of Cloning of 1793-pET28b-VPol1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-14----2013-8-16 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1793&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1793pcr1+pcr2 8-14-2013.JPG]]&lt;br&gt; <br /> <br /> Template: 1793-2 (2013/8/7) <br /> <br /> PCR1:1-1 to 2-4 <br /> <br /> PCR2:2-5 to 3-16 <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1793+1812PCR 8-14-2013.JPG]] <br /> <br /> template:pcr1+pcr2&lt;br&gt; <br /> <br /> VPol1-1-1+VPol1-3-16&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1793+1812clone 8-15-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;394&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1074,pET28b-IHFab&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | Nco1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-16 <br /> <br /> Make miniprep and digest with Nco1 + Xho1 <br /> <br /> [[Image:1793digest 8-16-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> 2013-8-30&lt;br&gt; <br /> <br /> [[Image:1793digest_8-30-2013.JPG]]<br /> <br /> [[Category:Cloning]]</div> Paul.xu