https://eln.excellgen.com/index.php?action=history&feed=atom&title=1794-pT7A32 2026-10-18T17:24:27Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1794-pT7A32&diff=9827&oldid=prev 2013-07-25T16:59:10Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1794-pT7A32&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1794-pT7A32 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-7-22---2013-7-25<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1794&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1794PCR 7-22-2013.JPG]]&lt;br&gt; <br /> <br /> gene synthesis <br /> <br /> Xba1-3c-tev-Xho1-1 to&amp;nbsp; Xba1-3c-tev-Xho1-6&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1794clone 7-23-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;306&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1279 pT7A3&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Xba1+Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-7-25<br /> <br /> Make miniprep and digest Xba1+Xho1<br /> <br /> [[Image:1794+1798_digest_7-25-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div>

Paul.xu