https://eln.excellgen.com/index.php?action=history&feed=atom&title=1797_1498_1781_1796_1497_1542 1797 1498 1781 1796 1497 1542 - Revision history 2026-10-18T02:16:03Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1797_1498_1781_1796_1497_1542&diff=10052&oldid=prev Paul.xu at 19:57, 15 August 2013 2013-08-15T19:57:54Z <p></p> <p><b>New page</b></p><div>== Protein Purification ==<br /> <br /> 2013/7/16&lt;br&gt; SHANYI <br /> <br /> [[Image:1797 1498 1781 1796 1497 1542.jpg]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;982&quot;<br /> |-<br /> | Lane&lt;br&gt; <br /> | Name&lt;br&gt; <br /> | Protein Name&lt;br&gt; <br /> | Clone ID&lt;br&gt; <br /> | Host&lt;br&gt; <br /> | &lt;br&gt; <br /> | Purification&lt;br&gt; <br /> | pI/Mw&lt;br&gt; <br /> | Expression &lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | rowspan=&quot;4&quot; | &lt;br&gt; &amp;nbsp; pCold-6His-3C-MBP&lt;br&gt; <br /> | rowspan=&quot;4&quot; | 3C-MBP&lt;br&gt; <br /> | rowspan=&quot;4&quot; | 1797&lt;br&gt; <br /> | rowspan=&quot;4&quot; | BL21(DE3)Star, pRare2-pLysS&lt;br&gt; <br /> | autoinduce&lt;br&gt; <br /> | rowspan=&quot;4&quot; | Ni beads&lt;br&gt; <br /> | rowspan=&quot;4&quot; | &lt;br&gt; 62KD&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | no IPTG&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | <br /> autoinduce&lt;br&gt; <br /> <br /> | rowspan=&quot;2&quot; | &lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | 2hIPTGinduce&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | <br /> pT7A3-TaqDNALigase&lt;br&gt; <br /> <br /> | TaqDNALigase&lt;br&gt; <br /> | 1498&lt;br&gt; <br /> | <br /> Nico21 DE3 plysN&lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | 10 ml Ni column <br /> Bio-Sil TSK 250&lt;br&gt; <br /> <br /> Q column&lt;br&gt; <br /> <br /> | <br /> 76KD&lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | pTCold-MBP-(3C-TEV)-PKARIIb&lt;br&gt; <br /> | MBP-(3C-TEV)-PKARIIb&lt;br&gt; <br /> | 1781&lt;br&gt; <br /> | <br /> Nico21(DE3)pRare2pLysS&lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | <br /> Ni column &lt;br&gt; <br /> <br /> Amylose column&lt;br&gt; <br /> <br /> | <br /> 5.11 / 93127.81&lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | pT7A3-MBP-(3C-TEV)-NRF2-6His&lt;br&gt; <br /> | NRF2&lt;br&gt; <br /> | 1796&lt;br&gt; <br /> | &amp;nbsp;Nico21(DE3)pRare2pLysS&lt;br&gt; <br /> | &lt;br&gt; <br /> | rowspan=&quot;1&quot; | Ni column <br /> Amylose column&lt;br&gt; <br /> <br /> TEVdigestion&lt;br&gt; <br /> <br /> | <br /> 4.67 / 68345.09 &lt;br&gt; <br /> <br /> | &lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | pT7A3-ToughTaq&lt;br&gt; <br /> | ToughTaq&lt;br&gt; <br /> | 1497&lt;br&gt; <br /> | &amp;nbsp; Nico21 DE3 plysN&lt;br&gt; <br /> | <br /> &amp;nbsp; 0.2mM IPTG,&lt;br&gt;<br /> <br /> 25c overnight<br /> <br /> | Ni column &lt;br&gt; <br /> | <br /> 72KD&lt;br&gt; <br /> <br /> | yes&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | pT7A3-sso7d-Native-ToughTaq&lt;br&gt; <br /> | ToughTaq&lt;br&gt; <br /> | 1542&lt;br&gt; <br /> | &amp;nbsp; BL21(DE3)Star, pRare2-pLysS&lt;br&gt; <br /> | &amp;nbsp; 0.2mM IPTG,<br /> 25c overnight<br /> <br /> &lt;br&gt;<br /> <br /> | Ni column &lt;br&gt; <br /> | 70KD&amp;nbsp; &lt;br&gt; <br /> | yes&lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | pT7A3-ToughTaq&lt;br&gt;<br /> | ToughTaq&lt;br&gt;<br /> | 1497&lt;br&gt; <br /> | &amp;nbsp; Nico21 DE3 plysN&lt;br&gt; <br /> | 0.2mM IPTG,<br /> 37c ,4h&lt;br&gt;<br /> <br /> | Ni column &lt;br&gt; <br /> | 72KD&lt;br&gt; <br /> | yes&lt;br&gt;<br /> |-<br /> | 12&lt;br&gt; <br /> | pT7A3-sso7d-Native-ToughTaq&lt;br&gt; <br /> | ToughTaq&lt;br&gt; <br /> | 1542&lt;br&gt; <br /> | BL21(DE3)Star, pRare2-pLysS&lt;br&gt; <br /> | 0.2mM IPTG,<br /> 37c ,4h&lt;br&gt;<br /> <br /> | Ni column &lt;br&gt; <br /> | &amp;nbsp; &lt;br&gt;70KD&lt;br&gt; <br /> | &amp;nbsp; yes&lt;br&gt;<br /> |}<br /> <br /> [[Category:Protein_Purification]]</div> Paul.xu