https://eln.excellgen.com/index.php?action=history&feed=atom&title=1798-pCold-6His-MCS-%28TEV-3C%29-MBP 1798-pCold-6His-MCS-(TEV-3C)-MBP - Revision history 2026-10-18T18:31:04Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1798-pCold-6His-MCS-(TEV-3C)-MBP&diff=9828&oldid=prev Paul.xu at 16:59, 25 July 2013 2013-07-25T16:59:59Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1798-pCold-6His-MCS-(TEV-3C)-MBP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1798-pCold-6His-MCS-(TEV-3C)-MBP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-7-22---2013-7-25<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1798&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1797pcr2+1798PCR+1800PCR 7-23-2013.JPG]]&lt;br&gt; <br /> <br /> PCR MBP using 1281 pMAL-p5E as template&lt;br&gt;MBP-3C-HindIII-F&lt;br&gt; MBP-Sal1-stop-R2 &lt;br&gt; <br /> <br /> [[Image:1798+1800clone 7-23-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;306&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | v19 pET28-cold&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | HindIII + Sal1&lt;br&gt; <br /> | HindIII + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-7-25<br /> <br /> Make miniprep and digest with HindIII + Bgl11<br /> <br /> [[Image:1794+1798_digest_7-25-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu