https://eln.excellgen.com/index.php?action=history&feed=atom&title=1802-pT7A3-Tth-Ligase-K294R
1802-pT7A3-Tth-Ligase-K294R - Revision history
2026-10-18T14:45:12Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=1802-pT7A3-Tth-Ligase-K294R&diff=9923&oldid=prev
Paul.xu: Created page with "== '''Cloning of 1802-pT7A3-Tth-Ligase-K294R''' == '''Purpose:''' Cloning of 1802-pT7A3-Tth-Ligase-K294R '''Date: '''2013-8-2----2013-8-5 '''Notebook:0''' '''Page:''' '..."
2013-08-05T17:15:24Z
<p>Created page with "== '''Cloning of 1802-pT7A3-Tth-Ligase-K294R''' == '''Purpose:''' Cloning of 1802-pT7A3-Tth-Ligase-K294R '''Date: '''2013-8-2----2013-8-5 '''Notebook:0''' '''Page:''' '..."</p>
<p><b>New page</b></p><div>== '''Cloning of 1802-pT7A3-Tth-Ligase-K294R''' ==<br />
<br />
'''Purpose:''' Cloning of 1802-pT7A3-Tth-Ligase-K294R <br />
<br />
'''Date: '''2013-8-2----2013-8-5 <br />
<br />
'''Notebook:0''' <br />
<br />
'''Page:''' <br />
<br />
'''Clone ID:1802''' <br />
<br />
'''Experimenters:''' yingying <br />
<br />
== '''Results''' ==<br />
<br />
=== cloning ===<br />
<br />
[[Image:1802+1804clone_8-2-2013.JPG]]<br> <br />
<br />
subclone from 1035 pET28b-Tth-Ligase-K294R to 1279 pT7A3 using Nde1 + EcoR1<br><br />
<br />
<br> <br />
<br />
<br> <br />
<br />
<br> <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2013-8-5 <br />
<br />
Make miniprep and digest with&nbsp; Nde1 + EcoR1<br />
<br />
[[Image:1802+1808digest_8-5-2013.JPG]]<br />
<br />
clone 5 for stock<br />
<br />
<br> <br />
<br />
<br> <br />
<br />
<br> <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
== '''Conclusion''' ==<br />
<br />
[[Category:Cloning]]</div>
Paul.xu