https://eln.excellgen.com/index.php?action=history&feed=atom&title=1805-pCold-MCS-pLacZ-3C 1805-pCold-MCS-pLacZ-3C - Revision history 2026-10-18T17:15:52Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1805-pCold-MCS-pLacZ-3C&diff=10033&oldid=prev Paul.xu at 15:50, 14 August 2013 2013-08-14T15:50:09Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1805-pCold-MCS-pLacZ-3C&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1805-pCold-MCS-pLacZ-3C <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-7---2013-8-14<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1805&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1805pcr1 8-7-2013.JPG]]&lt;br&gt; <br /> <br /> PCR lacZ promoter using pUC19 as template.&lt;br&gt;LacZ-Pro-BamH1-F&lt;br&gt;LacZ-Pro-3C-R&lt;br&gt; <br /> <br /> [[Image:1805pcr2 8-9-2013.JPG]]&lt;br&gt; <br /> <br /> 3C protease from 1420 pDB.His.MBP.3C&lt;br&gt;LacZ-Pro-3C-F&lt;br&gt;3C-Sph1-R&lt;br&gt; <br /> <br /> [[Image:1805PCR 8-9-2013.JPG]]&lt;br&gt; <br /> <br /> pcr1+pcr2&lt;br&gt; <br /> <br /> LacZ-Pro-BamH1-F+3C-Sph1-R&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1805+1812clone 8-12-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;427&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1755 pCold-6H&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | BamH1+Sph1&lt;br&gt; <br /> | BglII + Sph1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-14<br /> <br /> Make miniprep and digest&amp;nbsp; Sph1+Spe1&lt;br&gt; <br /> <br /> [[Image:1805digest_8-14-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu