https://eln.excellgen.com/index.php?action=history&feed=atom&title=1806-pCold-MBP-%28a3C%29-MCS-pLacZ-3C 1806-pCold-MBP-(a3C)-MCS-pLacZ-3C - Revision history 2026-10-18T16:31:27Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1806-pCold-MBP-(a3C)-MCS-pLacZ-3C&diff=10074&oldid=prev Paul.xu at 16:15, 19 August 2013 2013-08-19T16:15:56Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1806-pCold-MBP-(a3C)-MCS-pLacZ-3C&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1806-pCold-MBP-(a3C)-MCS-pLacZ-3C<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-16---2013-8-19<br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1806&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1806clone_8-16-2013.JPG]]&lt;br&gt;<br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; height=&quot;86&quot; width=&quot;478&quot;<br /> |-<br /> | lane&lt;br&gt;<br /> | 1&lt;br&gt;<br /> | 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | 1804, pCold-MBP-(E3C)&lt;br&gt;<br /> | 1805, pCold-MCS-pLacZ-3C&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1 + Nde1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;<br /> <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-19 <br /> <br /> Make miniprep and digest with &lt;br&gt; <br /> <br /> [[Image:1806digest 8-19-2013.JPG]]&lt;br&gt; <br /> <br /> clone 3 for stock&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; [[Category:Cloning]]</div> Paul.xu