https://eln.excellgen.com/index.php?action=history&feed=atom&title=1811-pT7A3-VPol1 1811-pT7A3-VPol1 - Revision history 2026-10-18T16:31:26Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1811-pT7A3-VPol1&diff=10309&oldid=prev Paul.xu at 19:55, 24 September 2013 2013-09-24T19:55:49Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1811-pT7A3-VPol1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1811-pT7A3-VPol1 <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-9-11--2013-9-13 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1811&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1811clone 9-11-2013.JPG]]&lt;br&gt; <br /> <br /> subclone from 1793 pET28b-VPol1 using Nco1 + Xho1&lt;br&gt; <br /> <br /> {| width=&quot;499&quot; border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 2&lt;br&gt; <br /> | 3&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | 1793 pET28b-VPol1&lt;br&gt; <br /> | 1279,pT7A3&lt;br&gt; <br /> | 1780,pT7A3-T7-RNAPol-Y639F&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | colspan=&quot;3&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-9-13 <br /> <br /> Make miniprep and digest with Nco1 + Xho1 <br /> <br /> [[Image:1811digest 9-13-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> == protein purification ==<br /> <br /> [[Image:1811protein_purification_9-24-2013.JPG]]<br /> <br /> <br /> <br /> [[Image:1811-2_protein_purification_9-24-2013.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Paul.xu