https://eln.excellgen.com/index.php?action=history&feed=atom&title=1812-pET28b-HTNFlp-Ecoli 1812-pET28b-HTNFlp-Ecoli - Revision history 2026-10-18T04:16:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=1812-pET28b-HTNFlp-Ecoli&diff=10252&oldid=prev Paul.xu: moved 1812-pEC-HTNFlp-Ecoli to 1812-pET28b-HTNFlp-Ecoli 2013-09-13T20:05:07Z <p>moved <a href="/index.php?title=1812-pEC-HTNFlp-Ecoli" class="mw-redirect" title="1812-pEC-HTNFlp-Ecoli">1812-pEC-HTNFlp-Ecoli</a> to <a href="/index.php?title=1812-pET28b-HTNFlp-Ecoli" title="1812-pET28b-HTNFlp-Ecoli">1812-pET28b-HTNFlp-Ecoli</a></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1812-pET28b-HTNFlp-Ecoli&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1812-pET28b-HTNFlp-Ecoli <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-9------2013-8-16 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1812&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:1812pcr1+2 8-7-2013.JPG]]&lt;br&gt; <br /> <br /> gene synthesis&lt;br&gt; <br /> <br /> pcr1:Ecoli-Flpe-1-1 to&amp;nbsp; Ecoli-Flpe-1-18&lt;br&gt; <br /> <br /> pcr2:Ecoli-Flpe-2-1 to Ecoli-Flpe-2-18&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1793+1812PCR 8-14-2013.JPG]]&lt;br&gt; <br /> <br /> pcr1+pcr2&lt;br&gt; <br /> <br /> PCR:Ecoli-Flpe-1-1 to Ecoli-Flpe-2-18 <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1793+1812clone 8-15-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;329&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 3&lt;br&gt; <br /> | 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1078,pET28b-scIHF2&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-16 <br /> <br /> Make miniprep and digest with Nco1 + Xho1 <br /> <br /> [[Image:1812digest 8-16-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Fix of Cloning of 1812-pET28b-HTNFlp-Ecoli&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 1812-pET28b-HTNFlp-Ecoli <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2013-8-20----2013-8-28 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:1812&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === cloning ===<br /> <br /> [[Image:556PCR+1793pcr1+1812pcr1 8-21-2013.JPG]] <br /> <br /> [[Image:556+1793+1812pcr2 8-20-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> gene synthesis&lt;br&gt; <br /> <br /> pcr1:Ecoli-Flpe-1-1 to&amp;nbsp; Ecoli-Flpe-2-6&lt;br&gt; <br /> <br /> pcr2:Ecoli-Flpe-2-7 to Ecoli-Flpe-2-18&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1793+1812PCR 8-26-2013.JPG]]&lt;br&gt; <br /> <br /> pcr1+pcr2&lt;br&gt; <br /> <br /> PCR:Ecoli-Flpe-1-1 to Ecoli-Flpe-2-18 <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:1812clone 8-26-2013.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; width=&quot;329&quot;<br /> |-<br /> | lane&lt;br&gt; <br /> | 1&lt;br&gt; <br /> | 3&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | 1078,pET28b-scIHF2&lt;br&gt;<br /> |-<br /> | enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1 + Xho1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2013-8-28 <br /> <br /> Make miniprep and digest with Nco1 + Xho1 <br /> <br /> [[Image:1812digest 8-28-2013.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> final fix&lt;br&gt; <br /> <br /> 2013-8-30 <br /> <br /> [[Image:1812digest 8-30-2013.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> FIX one A missing&lt;br&gt; <br /> <br /> 2013-9-6---2013-9-9 <br /> <br /> [[Image:1812digest 9-9-2013.JPG]] <br /> <br /> [[Category:Cloning]]</div> Paul.xu